摘要
一点缀螟Paralipsa gularis(Zeller)是近年来在云南玉米产区发现的一种严重危害玉米果穗的害虫,为了明确其肠道可培养细菌对食物营养及次生代谢物质的代谢功能,本研究采用透明圈生物测定法结合高效液相色谱技术,筛选研究了一点缀螟幼虫肠道可培养淀粉、纤维素、蛋白质、阿魏酸、香草醛和对羟基苯甲酸的降解菌.结果表明,从一点缀螟肠道可培养细菌中共筛选到18株分别具有淀粉、纤维素、蛋白质、阿魏酸、香草醛和对羟基苯甲酸降解力的细菌,其中淀粉降解能力最强的为枯草芽胞杆菌(DH3),透明圈直径(Hp)和菌落直径(Dp)的比值为1.70;纤维素的降解能力最强的是苏云金芽胞杆菌(BS1),Hp/Dp为4.46;蛋白质降解能力最强的为粘质沙雷氏菌(BS5),Hp/Dp为3.46;阿魏酸降解能力最强的是枯草芽胞杆菌(DH3),降解率达99.92％;香草醛的降解能力最强的是假格里尼翁布鲁氏菌(DH5),降解率达99.82％;腐生葡萄球菌(BS2和BS7)、平流层芽胞杆菌(BS4)、粘质沙雷氏菌(BS5)、蜡样芽胞杆菌(BS8)和假格里尼翁布鲁氏菌(DH5)的对羟基苯甲酸降解能力都很强,降解率均达100％.综上所述,一点缀螟肠道富含能降解玉米营养物质及玉米次生代谢物质的细菌,这些肠道细菌在一点缀螟的营养物质和次生代谢物质代谢中发挥了重要作用.该结果将为一点缀螟肠道细菌功能的研究及综合防治提供理论依据,同时为功能菌株的开发利用提供菌株资源.
Abstract
Paralipsa gularis(Zeller)(P.gularis)is a serious pest of corn cob found in Yunnan maize production ar-eas in recent years.To clarify the metabolic function of its intestinal culturable bacteria on food nutrients and secondary metabolites,we used the combination method of thylakoid bioassay and high-performance liquid chromatography,to screen and study the degrading bacteria of the larval intestine of P.gularis that can be cultured for starch,cellulose,pro-tein,ferulic acid,vanillin,and p-hydroxybenzoic acid.The results show that 18 bacterial strains are screened from P.gu-laris intestinal culturable bacteria with starch,cellulose,protein,ferulic acid,vanillin,and p-hydroxybenzoic acid degrad-ing power,respectively.Among them,Bacillus subtilis(DH3)has the strongest starch degrading ability,with the ratio of hyaline circle(Hp)and colony diameter(Dp)of 1.70.Bacillus thuringiensis(BS1)has the strongest degradation of cel-lulose,with Hp/Dp of 4.46.Serratia marcescens(BS5)has the strongest protein degradation,with Hp/Dp of 3.46.Ba-cillus.subtilis(DH3)has the strongest degradation of ferulic acid,with a degradation rate of 99.92％.Brucella pseu-dogrignonii(DH5)has the strongest degradation of vanillin,with a degradation rate of 99.82％.Staphylococcus sapro-phyticus(BS2 and BS7),Bacillus stratosphericus(BS4),Serratia marcescens(BS5),Bacillus cereus(BS8),and Bru-cella pseudogrignonensis(DH5)all have a strong ability to degrade p-hydroxybenzoic acid(degradation rates:100％).In summary,the intestinal tract of P.gularis is enriched with bacteria that can degrade maize nutrients and maize second-ary metabolites,and these intestinal bacteria play an important role in the metabolism of nutrients and secondary metabo-lites in P.gularis.The results will provide not only a theoretical basis for the study of the functions of P.gularis intestinal bacteria and the comprehensive control of P.gularis,but also the strain resources for development and utilization of func-tional bacterial strains.
维普
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