Progress in biosynthesis and key enzymes of Ala-Gln
L-alanyl-L-glutamine(Ala-Gln)is an important dipeptide in modern medical and health care.After en-tering the human body,it can be rapidly hydrolyzed into L-glutamine,which promotes protein synthesis.The synthesis of Ala-Gln dipeptide by biological method has the advantages of green,high efficiency and safety.The main biosynthetic pathways are microbial fermentation and biocatalysis,whose key enzymes are L-amino acid ligase and a-amino acid ester acyltransferase.The degradation of dipeptide can be inhibited by knocking out genes such as dpp and pep,and the highest level of Ala-Gln in the fermentation method can reach 24.70 g/L after a fermentation time of 47 h.a-amino acid ester ac-yltransferase synthesizes Ala-Gin from L-alanine methyl ester and L-glutamine,and the related research mainly focuses on the enzyme expression system,directional evolution,and immobilisation.a-amino acid ester acyltransferase can con-vert and produce 106.61 g/L of Ala-Gln in 25 min.Compared with microbial fermentation,this method has a shorter re-action time,higher product concentration,and does not need ATP for energy supply,making it the preferred process technology for industrial production of Ala-Gln.
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