Banana tissue culture and rapid propagation using intermittent immersion plant bioreactor
Banana sprouts were used as experimental materials to compare the rapid propagation of banana tissue cul-ture using intermittent immersion plant bioreactors and traditional tissue culture method.Banana seedlings were cultured by the two cultivation methods,and then the growth morphology,multiplication ratio and photosynthetic index of banana seedlings were compared.The single-factor experimental analysis method was used to optimize immersion frequency,and chlorophyll content was measured by using UV spectrophotometry.The results showed that the immersion frequency of 20 min/6 h is the optimal culture condition for rapid propagation of bananas in the plant bioreactor.And the growth morphology,multiplication rate,chlorophyll content,and photosynthetic performance of banana tissue cultured seedlings are significantly different between the two cultivation methods.The use of the intermittent immersion plant bioreactors can significantly increase the multiplication rate of banana tissue cultured seedlings and improve their overall growth.Ad-ditionally,the cultivation using the intermittent immersion plant bioreactors enhances chlorophyll content as well as photo-synthetic performance.
bananaplant tissue cultureplant bioreactorimmersion frequency
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