球腔菌属菌株(Mycosphaerella sp.HS-47)发酵产物中具有抗菌活性的麦角甾的分离与鉴定
Isolation and identification of ergosterols with antimicrobial activity in the fermentation products of Mycosphaerella sp.HS-47
潘超 1花思露 1刘萍 1缪莉 1姜薇1
作者信息
- 1. 扬州大学环境科学与工程学院,江苏扬州 225127
- 折叠
摘要
从源自红树根际土壤的一种球腔菌属菌株Mycosphaerella sp.HS-47发酵产物中分离出具有抗菌活性的次生代谢产物.综合运用多种色谱方法,从乙酸乙酯萃取物中分离得到7个化合物,将其与波谱数据进行比对,这7个化合物分别被鉴定为 ergost-7,9(11),22-trien-3β,5α,6α-triol(1);ergost-7,22-diene-3β,5α,6α-triol(2);6,9-epoxy-ergosta-7,22-dien-3-ol(3);3β,5α,9α-trihydroxy-(22E,24R)-ergosta-7,22-dien-6-one(4);22E-7α-methoxy-5α,6α-epoxyergosta-8(14),22-dien-3β-ol(5);5α,6α-epoxy-(22E,24R)-ergosta-8,22-dien-3β,7α-diol(6)和 3β,5α-dihydroxy-10α-methyl-6p-acetoxy-ergosta-7,22-diene(7).抗菌活性结果表明,化合物1～7具有抗菌活性,其中化合物5和化合物6活性最为显著,对耐甲氧西林金黄色葡萄球菌S.aureus ATCC43300的最小抑制浓度均为1.560 μg/mL.该研究首次从球腔菌属菌株HS-47中分离出的甾体类化合物,其具有不同程度的抗菌活性.
Abstract
The secondary metabolites with antimicrobial activity were isolated from the fermentation products of My-cosphaerella sp.HS-47 derived from mangrove rhizosphere soil.Seven compounds were obtained from the EtOAc extract by means of various chromatographic techniques.The structures of the seven compounds were identified as ergost-7,9(11),22-trien-3β,5α,6α-triol(1);ergost-7,22-diene-3β,5α,6α-triol(2);6,9-epoxy-ergosta-7,22-dien-3-ol(3);3β,5α,9α-trihydroxy-(22E,24R)-ergosta-7,22-dien-6-one(4);22E-7α-methoxy-5α,6α-epoxyergosta-8(14),22-dien-3β-ol(5);5α,6α-epoxy-(22E,24R)-ergosta-8,22-dien-3β,7α-diol(6),and 3β,5α-dihydroxy-10α-meth-yl-6β-acetoxy-ergosta-7,22-diene(7),respectively,by comparison with spectroscopic data in the literature.The results of antimicrobial activity showed that compounds 1～7 have antimicrobial activity.Compound 5 and compound 6 have the most significant activity,with a minimum inhibitory concentration of 1.560 μg/mL for methicillin-resistant S.aureus ATCC43300.The ergosterols isolated from Mycosphaerella sp.HS-47 are reported for the first time,and they have varying degrees of antimicrobial activity.
关键词
球腔菌属菌株HS-47/麦角甾/分离与鉴定/抗菌活性Key words
Mycosphaerella sp.HS-47/ergosterols/isolation and identification/antimicrobial activity引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据