Effect of AMF and PGPR combined with Phragmites australis on the remediation of heavy metal polluted river sediment
The objective of this experiment is to assess the impacts of Arbuscular Mycorrhizal Fungi(AMF)and Plant Growth Promoting Rhizobacteria(PGPR)on the growth,phosphorus uptake,and heavy metal removal of reeds in river sediment affected by heavy metal pollution.Utilizing sediment from the Hunhe River in Shenyang City as the subject of study,six treatments were administered to reeds,which were inoculated with AMF Acalospora bioreticulata(AM1),Funneliformes mosesae(AM2),PGPR Serratia marcescens(PG),APG1,APG2,and a control group(CK).The findings revealed that AMF can enhance the colonization of PGPR in the rhizosphere soil and reed roots,while PGPR can foster the advancement of AMF infection,leading to an increase in its infection rate,vesicle number,and spore count.Treatments involving AMF,PGPR,or a combination of both significantly amplified reed biomass and the ratio of aboveground and root phosphorus mass,thereby facilitating the uptake of heavy metals(Cd,Zn,Pb,Cu)from the sediment by plants and reducing the heavy metal content in the soil.In each treatment,the APG2 treatment exhibited the highest heavy metal removal rate from the sediment,ranging from 33.24％to 55.51％.Moreover,the soil's catalase,sucrase,urease,and alkaline phosphatase activities were notably higher in comparison to the AM1,AM2,PG,and CK treatments.Furthermore,the distribution of heavy metals within the reed body indicated a greater presence in the root system than in the aboveground portion,suggesting that the reed's root system possesses a more robust capacity for absorbing and accumulating heavy metals.Furthermore,the APG2 treatment exhibited the most effective absorption of Cd,Zn,and Pb,surpassing the CK treatment by 2.4,2.0,and 1.7 times,respectively.The findings suggest that the simultaneous inoculation of AMF and PGPR can enhance phosphorus absorption and soil enzyme activity in reeds,fostering plant growth.Moreover,the synergy between AMF and PGPR can mutually facilitate and elevate the efficiency of heavy metal removal from sediment.Notably,the APG2 combination emerged as the top-performing treatment in this experiment.
万方数据
维普
