Cysteamine-modified copper complexes for sensitive detection of chloramphenicol
To enhance the sensitivity and stability of the fluorometric detection of the antibiotic chlorampheni-col(CAP),a fluorescent probe of copper complex modified with cysteamine(CuCy-Cl)was prepared.The experimental results indicated that CuCy-Cl exhibited excellent fluorescence stability and demonstrated high selectivity and outstanding sensitivity to chloramphenicol.Based on the dynamic quenching mechanism,the effective identification of antibiotic chloramphenicol was realized.In the range of 0.5～9 µmol/L or 10～90 µmol/L,F0/F showed a good linear relationship with CCAP,and the detection limit was 0.128 µmol/L.CuCy-Cl was successfully used for the fluorescence determination of chloramphenicol in lake water and eye drops with recoveries of 97.4%～103.2%.
万方数据
维普
