Optimization of Extraction Process of Tea Polysaccharide from Oolong Tea Based on Response Surface Methodology
With oolong tea as the raw material,the tea polysaccharide was extracted by reflux method.Accord-ing to Plackett-Burman experiment,the effects of extraction temperature,extraction duration,extraction fre-quency and solid-liquid check on the extraction rate of tea polysaccharide were studied.Under the premise of single factor experiment,the box Behnken experiment is designed and the response surface is analyzed.The re-sults showed that when the extraction temperature was 100℃,the extraction period was 80 min,and the ratio of solid to liquid was 1∶50,the extraction rate of oolong tea polysaccharide was significantly increased,and the yield was 6.13%.The best extraction processing technology is proved,and the evaluation mean value is close to the estimated value of the entity model,indicating that the extraction processing technology is stable and fea-sible.
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