Differential microRNA of lymphoid specific helicase overexpression lung adenocarcinoma cell and effect of its key gene on prognosis based on public database screening
Objective To analyze the expression difference of microRNA(miRNA)after overexpression of lymphoid specific helicase(LSH)in lung adenocarcinoma PC9 cells,and to predict its potential regulatory genes.Method The total miRNA in LSH-overexpressed PC9 cell lines were detected by high-throughput gene sequencing technology,and candi-date miRNA with different nucleoplasmic ratios were screened,and the nucleoplasmic ratios of candidate miRNA were verified by real-time quantitative polymerase chain reaction(PCR).The Kyoto Encyclopedia of Genes and Genomics(KEGG)enrichment analysis and Gene Ontology(GO)functional enrichment analysis were performed on regulatory genes by bioinformatics methods,and protein-protein interaction(PPI)networks were constructed to screen miRNA nu-clear targets.The effects of candidate target genes on the prognosis of lung adenocarcinoma patients were investigated by The Cancer Genome Atlas(TCGA)database.Result By sequencing and gene difference analysis of LSH-overexpressed PC9 cells,five miRNA with different nucleoplasmic ratio were screened:hsa-miRNA-30e-5p,hsa-miRNA-378i,hsa-miR-NA-378f,hsa-miRNA-423-5p,hsa-miRNA-4284,among which the increase of nuclear miRNA-4284 was the most signif-icant.KEGG and GO enrichment analysis showed that these miRNA might be involved in the regulation of RAS signal-ing pathway by binding target genes.Through PPI networks and lung cancer prognosis prediction,differential miRNA down-regulate the expression of C-X-C motif chemokine receptor 4(CXCR4)and increased the death risk of lung adeno-carcinoma patients.Conclusion LSH-overexpressed lung adenocarcinoma PC9 has multiple miRNA with significantly different expression in the nucleus and cytoplasm,and the change of miRNA-4248 in the nucleus is the most obvious.Bioinformatics analysis shows that different miRNA may increase the death risk of lung adenocarcinoma patients by down-regulating CXCR4 expression.
lung cancerlymphoid specific helicasemicroRNAmiRNA-4248C-X-C motif chemokine receptor 4
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