Relationship between the expression level of long non-coding RNA epidermal growth factor receptor-antisense RNA 1 in lung adenocarcinoma and the therapeutic efficacy and prognosis of epidermal growth factor receptor-tyrosine kinase inhibitor
Objective To investigate the relationship between the expression level of long non-coding RNA(ln-cRNA)epidermal growth factor receptor(EGFR)-antisense RNA 1(AS1)in lung adenocarcinoma and the therapeutic ef-ficacy and prognosis of EGFR-tyrosine kinase inhibitor(TKI).Method Real time fluorescence quantitative polymerase chain reaction(PCR)was used to detect and compare the expression levels of lncRNA EGFR-AS1 in lung adenocarcino-ma tissues and paracancer tissues of 80 lung adenocarcinoma patients,three groups of transfected PC9 cells(control group,pcDNA3.1-NC group,pcDNA3.1-EGFR-ASl group),PC9 and PC9/GR cells cultured with gefitinib.The viability and migration ability of cells in three groups of transfected PC9 cells were detected by methyl thiazolyl terazolium(MTT)and cell scratch assay,respectively.Lung adenocarcinoma patients were divided into lncRNA EGFR-AS1 high expression group and lncRNA EGFR-AS1 low expression group according to the median expression level of lncRNA EGFR-AS1.The effect of EGFR-TKI treatment,the relationship expression level of lncRNA EGFR-AS1 and clinical characteristics,the survival were compared between the lncRNA EGFR-AS1 high and low expression groups.Cox regression was used to analyze the prognostic factors of lung adenocarcinoma patients.Result The expression levels of lncRNA EGFR-AS1 in lung adenocarcinoma tissues and PC9/GR cells cultured by gefitinib were higher than those in paracancer tissues and PC9 cells,and the differences were statistically significant(P＜0.05).The total effective rate and median survival time of EGFR-TKI treatment in lncRNA EGFR-AS1 low expression group were significantly higher than those in lncRNA EGFR-AS1 high expression group,and the differences were statistically significant(P＜0.01).The TNM stage,differentiation de-gree,lymph node metastasis,and Eastern Cooperative Oncology Group(ECOG)performance status score in lncRNA EG-FR-AS1 high and low expression groups were compared,and the differences were statistically significant(P＜0.05).The results of multivariate Cox analysis showed that TNM stage Ⅲ-Ⅳ and high expression of lncRNA EGFR-AS1 were risk factors for poor prognosis in lung adenocarcinoma patients(P＜0.05).The expression level of lncRNA EGFR-AS1,cell survival rate and migration rate in PC9 cells of the pcDNA3.1-EGFR-AS1 group were higher than those in control group and pcDNA3.1-NC group,and the differences were statistically significant(P＜0.05).Conclusion lncRNA EGFR-AS1 is involved in the occurrence and development of lung adenocarcinoma,and affects the prognosis of patients and the effec-tiveness of EGFR-TKI treatment.
万方数据
维普
