Screening for prognostic-related genes of lung adenocarcinoma based on Gene Expression Omnibus and The Cancer Genome Atlas databases
Objective To screen prognostic-related genes of lung adenocarcinoma(LUAD)based on Gene Expres-sion Omnibus(GEO)and The Cancer Genome Atlas(TCGA)databases.Method Three datasets GSE118370,GSE10072 and GSE115002 were selected from GEO database.R software was usd to screen differential genes in LU AD tissues and adjacent normal lung tissues,Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed for differential genes.STRING database was used to construct protein-pro-tein interaction(PPI)networks associated with differential genes.The key genes in differential genes were identified by the software Cytoscape,and the expression of key genes in LUAD tissues and normal lung tissues were analyzed by Gene Expression Profiling Interactive(GEPIA)database.The overall survival and disease-free survival of LUAD patients with high and low expression of key genes were compared.The expression of key genes in LUAD patients with different clini-cal stages were compared.UALCAN database was used to analyze the expression of key genes in LU AD tissues and nor-mal lung tissues.Result A total of 101 up-regulated differential genes and 213 down-regulated differential genes were obtained.GO enrichment analysis showed that up-regulated differential genes were mainly enriched in biological process-es such as extracellular matrix tissues,mitotic cell cycle,epithelial cell differentiation,as well as molecular functions such as cell adhesion molecule binding and protein kinase binding.KEGG pathway enrichment analysis showed that up-regulated differential genes were mainly enriched in pathway such as p53 signaling pathway and relaxin signaling path-way.GO enrichment analysis showed that down-regulated differential genes were mainly enriched in biological processes such as vascular development,cell response to cytokine stimulation,and cellular components such as extracellular matrix and membrane rafts.The KEGG pathway enrichment analysis showed that down-regulated differential genes were mainly enriched in pathways such as cytokine-cytokine receptor interaction and neuroactive ligand-receptor interaction.Finally,eight key genes were selected,which were BUB1 mitotic checkpoint serine/threonine kinase B(BUB1B),ZW10 interact-ing kinetochore protein(ZWINT),cyclin dependent kinase 1(CDK1),DNA topoisomerase Ⅱ alpha(TOP2A),ubiquitin conjugating enzyme E2C(UBE2C),cyclin B2(CCNB2),NIMA related kinase 2(NEK2),assembly factor for spindle mi-crotubules(ASPM).The expression levels of these 8 genes in LUAD tissues were higher than those in normal lung tis-sues,and were related to the overall survival,disease-free survival and clinical stage of LUAD patients.Conclusion BUB 1B,ZWINT,CDK1,TOP2A,UBE2C,CCNB2,NEK2,ASPM genes are differentially expressed in LUAD tissues and normal lung tissues,and correlated with overall survival,disease-free survival and clinical stage of LUAD patients.
万方数据
维普
