Objective To investigate the relationship between mitochondrial ribosomal protein L3(MRPL3)and pro-liferation,migration of lung cancer cells.Method The expression levels of MRPL3 in tumor tissues and adjacent tissues were obtained from The Cancer Genome Atlas(TCGA)database and UALCAN.The overexpressing MRPL3 lentivirus transfected H1299 cell line was constructed,and the knockdown MRPL3 lentivirus transfected HCC827 cell line was con-structed.Quantitative reverse transcription-polymerase chain reaction(qRT-PCR)and Western blot were used to verify the successful construction of cell lines or not.The proliferative and migration ability of lung cancer cells were detected by CCK-8 assay and Transwell cell migration assay.Result The bioinformatics analysis showed that the expression lev-el of MRPL3 in lung adenocarcinoma tissues was higher than that in adjacent normal tissues,and the expression levels of MRPL3 in various tumor tissues were generally higher than those in adjacent tissues.The proliferation and migration abil-ity of overexpressing MRPL3 lung cancer cells were higher than those of control group,and the proliferation and migra-tion ability of knockdown MRPL3 lung cancer cells were lower than those of control group.Conclusion Overexpression of MRPL3 can promote the proliferation and migration of lung cancer cells.
mitochondrial ribosomal protein L3lung cancerproliferationmigration
NETL
NSTL
万方数据
