Objective To investigate the screening value of human papilloma virus(HPV)DNA,E6/E7 mRNA,and thin-prep cytology test(TCT)for female cervical cancer.Method A total of 28 021 women who underwent physical ex-amination were selected,and all subjects received single or multiple tests for HPV DNA,E6/E7 mRNA,and TCT detec-tion.The detection rate of cervical cancer by HPV DNA,E6/E7 mRNA,and TCT single or combined detection were re-corded.The tissue biopsy results as the gold standard,the screening value of HPV DNA,E6/E7 mRNA,and TCT single or combined detection for cervical cancer were evaluated.Result The detection rates of TCT,HPV DNA,E6/E7 mRNA for cervical cancer were 8.8%,26.9%,and 29.7%,respectively.When each method single detection,the specificity(85.53%)for screening cervical cancer of TCT single detection was the highest,but the sensitivity(28.44%)was the low-est,the sensitivity(60.25%)for screening cervical cancer of HPV DNA single detection was the highest.When any two methods combined detection,the sensitivity(81.75%)for screening cervical cancer of HPV DNA+E6/E7 mRNA com-bined detection was the highest,the specificity(61.08%)and negative predictive value(57.11%)for screening cervical cancer of TCT+HPV DNA combined detection were the highest.The sensitivity and specificity of any two methods com-bined detection for screening cervical cancer were compared,the differences were statistically significant(P<0.05).The sensitivity and specificity of TCT+HPV DNA+E6/E7 mRNA and HPV DNA+E6/E7 mRNA combined detection for screening cervical cancer were not significantly differences(P>0.05),but the sensitivity of TCT+HPV DNA+E6/E7 mRNA was higher than that of TCT+E6/E7mRNA combined detection,the specificity was lower than that of TCT+E6/E7 mRNA combined detection,and the differences were statistically significant(P<0.05).Conclusion HPV DNA testing is suitable as the preferred method for cervical cancer screening in female health checkups,while E6/E7 mRNA and TCT testing can be preferred for further examination of cervical cancer.The combined detection of HPV DNA+E6/E7 mRNA has high sensitivity and positive predictive value,and can be used as the preferred screening method for cervical cancer in young women.The combined detection is helpful in improving the positive rate and reducing false positive rate.
human papilloma virus DNAE6/E7 mRNAthin-prep cytology testcervical cancerhuman papilloma virus
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NSTL
万方数据
