摘要
目的:探讨circFOXK2靶向miR-4677-3p对口腔鳞癌(OSCC)细胞恶性行为的影响.方法:RT-qPCR检测circFOXK2和miR-4677-3p在OSCC组织和癌旁组织的表达.分别将si-circFOXK2、si-NC、miR-NC、miR-4677-3p模拟物、pcDNA、pcDNA-circFOXK2、si-circFOXK2+anti-miR-NC、si-circFOXK2+anti-miR-4677-3p转染OSCC细胞HSC3.通过划痕愈合实验、CCK-8法、Transwell实验、集落形成实验检测circFOXK2和miR-4677-3p表达对HSC3细胞恶行行为的影响.circFOXK2和miR-4677-3p的靶向关系通过双荧光素酶法确定.结果:OSCC组织中circFOXK2表达水平显著高于癌旁组织(P<0.01),miR-4677-3p表达水平显著低于癌旁组织(P<0.01).抑制circFOXK2表达后HSC3细胞吸光度(OD)值、划痕愈合率、克隆形成数、侵袭数显著降低(P<0.01),miR-4677-3p表达水平显著升高(P<0.01).过表达miR-4677-3p后HSC3细胞OD值、划痕愈合率、克隆形成数、侵袭数显著降低(P<0.01).miR-4677-3p是circFOXK2的靶基因.下调miR-4677-3p显著减弱抑制circFOXK2表达对HSC3细胞OD值、划痕愈合率、克隆形成数、侵袭数的影响(P<0.01).结论:抑制circFOXK2可通过促进miR-4677-3p表达来抑制OSCC细胞增殖、迁移和侵袭.
Abstract
Objective:To study the effect of circFOXK2 targeting miR-4677-3p on the malignant behavior of oral squamous cell carcinoma ( OSCC) cells. Methods:The expression of circFOXK2 and miR-4677-3p in OSCC tissues and adjacent tissues was calculated by RT-qPCR. Si-circFOXK2,si-NC,miR-NC,miR-4677-3p mimic,pcDNA,pcDNA-circFOXK2,si-circFOXK2 +anti-miR-NC,and si-circFOXK2+anti-miR-4677-3p were respectively transfected into OSCC cells HSC3. The effects of circFOXK2 and miR-4677-3p expression on the malignant behavior of HSC3 cells were detected by scratch healing,CCK-8,Transwell and colony formation assays. The targeting relationship between circFOXK2 and miR-4677-3p was determined by dual luciferase method. Results:The expression level of circFOXK2 in OSCC tissue was significantly higher than that in adjacent tissues (P<0. 01),while the expression level of miR-4677-3p was significantly lower than that in adjacent tissues (P<0. 01). After inhibiting the expression of circFOXK2,the HSC3 cell optical density ( OD) value,scratch healing rate,clone formation number and invasion number were significantly reduced (P<0. 01),and the expression level of miR-4677-3p was significantly increased (P<0. 01). After overexpression of miR-4677-3p,the HSC3 cell OD value,scratch healing rate,clone formation number and invasion number were significantly reduced (P<0. 01). MiR-4677-3p was a target gene of circFOXK2. MiR-4677-3p down-regulation significantly reduced the effect of inhibiting circFOXK2 on cell OD value,scratch healing rate,clone formation number,and number of invasion of HSC3 cells (P<0. 01). Conclusions:Inhibition of circFOXK2 can suppress the proliferation,migration and invasion of OSCC cells by promoting miR-4677-3p expression.
维普
万方数据