99Tcm-DTPA-CGRRAGGSC在非小细胞肺癌裸鼠模型的显像研究
Imaging study on the 99Tcm-DTPA-CGRRAGGSC scintigraphy in the nude mice model with non-small cell lung cancer
李卫鹏 1李辉 1刘春艳 2沈如月 1訾刚 1袁超1
作者信息
- 1. 蚌埠医科大学第一附属医院 核医学科,安徽蚌埠 233004
- 2. 安徽省蚌埠市中医院 导管室,233080
- 折叠
摘要
目的:探讨放射性核素99Tcm标记环九肽(CGRRAGGSC)对荷非小细胞肺癌裸鼠的显像研究.方法:用99Tcm标记DTPA-CGRRAGGSC,测定其标记率及稳定性,制备荷非小细胞肺癌小鼠模型,经荷瘤鼠尾静脉注射标记物2 h后进行SPECT静态显像,并计算出靶与非靶(T/NT)比值.结果:成功制备出99Tcm-DTPA-CGRRAGGSC,在PBS和人血清中37 ℃放置6 h时放射性化学纯度在90％以上;经荷瘤鼠尾静脉注射标记物2 h后T/NT比值最高达3.5.结论:99Tcm标记DTPA-CGRRAGGSC制备简便,其对荷非小细胞肺癌裸鼠具有较高靶向性,99Tcm-DTPA-CGRRAGGSC有可能在IL-11Rα高表达的非小细胞肺癌分子显像中发挥重要作用.
Abstract
Objective:To explore the imaging of scintigraphy of CGRRAGGSC labeled with 99Tcm in nude mice with non-small cell lung cancer.Methods:The DTPA-CGRRAGGSC was labeled with 99Tcm,and the labeling rate and stability were measured.The mice model with non-small cell lung cancer were established.The markers were injected into the tail vein of the tumor-bearing mice,the SPECT static imaging was performed after 2 hours,and the ratio of target to non-target(T/NT)was calculated.Results:The 99Tcm-DTPA-CGRRAGGSC was successfully radiolabeled,and the radiochemical purity of which was above 90％ when it was placed in PBS and human serum at 37℃ for 6 h.The T/NT ratio was as high as 3.5 after 2 hours of injection of the marker through the tail vein of tumor-bearing mice.Conclusions:The 99Tcm-labeled DTPA-CGRRAGGSC is easy to be prepared,and it has high targeting to nude mice with non-small cell cancer.99Tcm-DTPA-CGRRAGGSC may play an important role in molecular imaging of non-small cell lung cancer with high expression of IL-11Rα.
关键词
肺肿瘤/99Tcm标记环九肽/白细胞介素-11Rα/放射性核素标记Key words
lung neoplasms/99Tcm-DTPA-CGRRAGGSC/interleukin-11Rα/radionuclide labeling引用本文复制引用
基金项目
蚌埠医学院自然科学研究重点项目(2020byzd097)
出版年
2024
NETL
NSTL
万方数据