MATN3的表达对胃癌细胞侵袭迁移能力的影响及EMT的相关性研究

Effect of MATN3 expression on invasion and migration ability of gastric cancer cells and correlation study of EMT

李靖 ¹吴永友 ²谢波 ³喻大军 ³汪虎³

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作者信息

1. 蚌埠医科大学第一附属医院肿瘤外科,安徽蚌埠 233004;苏州大学附属第二医院胃肠外科,江苏苏州 215000
2. 苏州大学附属第二医院胃肠外科,江苏苏州 215000
3. 蚌埠医科大学第一附属医院肿瘤外科,安徽蚌埠 233004
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摘要

目的:探讨胃癌细胞中人母系蛋白3(MATN3)的表达变化对胃癌细胞侵袭迁移能力及上皮间质转化(EMT)的影响,方法:检测不同胃癌细胞株中的MATN3 的表达,并检测MATN3 的沉默和过表达效果,及沉默和过表达MATN3 后的胃癌细胞侵袭迁移能力的变化、胃癌细胞 EMT 相关蛋白的表达变化.结果:MATN3 在不同胃癌细胞株中的表达存在差异性;沉默MATN3,胃癌细胞的侵袭迁移能力明显降低(P＜0.01),过表达MATN3,胃癌细胞的侵袭迁移能明显增强(P＜0.01);沉默MATN3,EMT相关蛋白E-Cadherin的表达降低,N-Cadherin的表达升高(P＜0.01);过表达MATN3,E-Cadherin的表达升高,N-Cadherin的表达降低(P＜0.01).结论:MATN3 的表达变化导致了胃癌细胞的侵袭迁移能力的改变,并导致EMT相关蛋白表达改变,从而影响了胃癌细胞的EMT过程.

Abstract

Objective:To investigate the effect of martrilin3(MATN3)expression on invasion and migration ability and epithelial mesenchymal transition(EMT)of gastric cancer cells.Methods:The expression of MATN3 in different gastric cancer cell lines was detected.The efficiency of silencing and overexpressing MATN3 was assessed.The changes of invasion and migration ability,and expression of EMT-related proteins of gastric cancer cells after silencing and overexpressing MATN3 were detected.Results:There were differences in the expression of MATN3 in different gastric cancer cell lines.Silencing MATN3 resulted in a decrease in the invasion and migration ability of gastric cancer cells(P＜0.01),while overexpression of MATN3 resulted in an increase in the invasion and migration ability of gastric cancer cells(P＜0.01).Silencing MATN3 led to decreased expression of EMT-related protein E-Cadherin and increased expression of N-Cadherin,while overexpression of MATN3 led to increased expression of E-Cadherin and decreased expression of N-Cadherin(P＜0.01).Conclusions:The change of MATN3 expression leads to the change of invasion and migration ability of gastric cancer cells and the change of EMT-related protein expression,which affects the EMT process of gastric cancer cells.

关键词

胃肿瘤/人母系蛋白3/侵袭迁移/上皮间质转化

Key words

gastric neoplasms/martrilin3/invasion and migration/epithelial mesenchymal transition

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基金项目

蚌埠医学院自然科学研究重点项目(2022byzd029)

安徽省高校自然科学研究重点项目(KJ2021A0729)

出版年

2024

蚌埠医学院学报

蚌埠医学院

蚌埠医学院学报

CSTPCD

影响因子：0.917

ISSN：1000-2200

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