摘要
目的:探讨成洞龋病人中唾液代谢物的表达及其与龋活性的关系.方法:选取成洞龋病人10 例(成洞龋组),健康无龋者10 例(无龋组),收集相应的唾液样本,基于质谱检测唾液代谢物的水平,比较2 组间是否存在差异代谢物(差异倍数≥2,变量投影重要度≥1,P＜0.05),及差异代谢物的诊断效能,采用MetOrigin和KEGG分别分析代谢物的起源和功能.结果:在2 组人群唾液样本中共检测到901 种代谢物,代谢物起源包括宿主、微生物、药物、食物、环境或共代谢.与无龋组相比,成洞龋组呈明显不同的代谢物表达谱.2-[(2-乙基己基)氧基]羰基苯甲酸和L-高瓜氨酸在成洞龋组为高表达(差异倍数=29707和5057,P＜0.01);而草酰乙酸、2-苯基乙酰胺、DL-苯甘氨酸,谷氨酸—亮氨酸—精氨酸在成洞龋组均为低表达(P＜0.01),差异倍数均高于1000,AUC值高于0.9.差异代谢物参与微生物的代谢及次级代谢产物的形成.结论:成洞龋组与无龋组存在不同的代谢表达谱,2-[(2-乙基己基)氧基]羰基苯甲酸和L-高瓜氨酸与成洞龋的发生呈正相关关系,而草酰乙酸、2-苯基乙酰胺、DL-苯甘氨酸、谷氨酸—亮氨酸—精氨酸与成洞龋的发生呈负相关关系.
Abstract
Objective:To explore the expression of salivary metabolites in patients with cavitated caries,and their relationship with caries activity.Methods:Ten cases of saliva samples were collected from 10 patients with cavitated caries cavitated group,and 10 health cases were selected as control group.The saliva samples in two groups were collected.The levels of salivary metabolites were detected by mass spectrometry to compare whether there were different metabolites between two groups(fold change≥2,VIP≥1,P＜0.05)and diagnostic efficiency of the different metabolites.The MetOrigin and KEGG were used to analyze the origin and function of metabolites,respectively.Results:A total of 901 metabolites were detected in the saliva samples of two groups,and their origin included the host,microbe,drug,food,environment or co-metabolism.Compared with the caries-free group,the Exp group showed significantly different metabolite profiles.The 2-((2-ethylhexyl)oxy)carbonyl benzoic acid and L-homocitrulline were highly expressed(FC=29707 and 5057,P＜0.01),the expression levels of oxaloacetic acid,2-phenylacetamide,DL-phenylglycine and glutamine-leucine-arginine were low in the Exp group(P＜0.01),the difference multiple was higher than 1000,and the AUC value was higher than 0.9.The differential metabolites participated in the microbial metabolism and formation of secondary metabolites.Conclusions:There are different metabolic expression profiles between the Exp group and control group.The 2-((2-Ethylhexyl)oxy)carbonyl benzoic and L-homocitrulline are positively correlated with the caries,while the oxaloacetic acid,2-phenylacetamide,2-phenylglycine,Glu-Leu-Arg are negatively correlated with caries.
基金项目
安徽省高校科学研究优秀青年项目(2023AH030088)
安徽省高校协同创新项目(GXXT-2021-056)
蚌埠医学院第一附属医院优秀青年项目(2019byyfyyq07)
蚌埠医学院自然科学研究重点项目(2021byzd133)
维普
万方数据