中国中南部G8P[8]型A组轮状病毒全基因组遗传特征研究
Genome-wide Molecular Characterization of G8P[8]-type Group-A Rotavirus in South-central China
熊光萍 1田毅 2范佳欣 3唐晓苹 1董梦洁 3车如意 3李伟红 4唐梅荣 5周珺 6刘颂 7王铭月 1李丹地 3段招军3
作者信息
- 1. 甘肃中医药大学公共卫生学院,兰州 730000;传染病溯源预警与智能决策全国重点实验室,中国疾病预防控制中心病毒病预防控制所,北京 102206
- 2. 沧州医学高等专科学校病原生物学与免疫学教研室,沧州 061001
- 3. 传染病溯源预警与智能决策全国重点实验室,中国疾病预防控制中心病毒病预防控制所,北京 102206
- 4. 北京市疾病预防控制中心,北京 100013
- 5. 广西壮族自治区疾病预防控制中心,南宁 530028
- 6. 江西省疾病预防控制中心,南昌 330046
- 7. 青岛市疾病预防控制中心,青岛 266033
- 折叠
摘要
G8基因型轮状病毒已在我国出现并迅速增加.本研究拟对采集于中国中南部5岁以下急性胃肠炎患儿的7例G8P[8]型RVA毒株进行全基因组分子特征分析.对轮状病毒特异性扩增结合高通量测序方法对中国中南部14 株 G8P[8]型 RVA 毒株进行全基因组测序.利用MEGA11.0、Geneious Prime、Geneious9.0.2和DNASTAR软件对RVA毒株进行全序列及遗传变异和进化分析.7株G8P[8]型RVA的基因分型为G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E1-H2,显示为DS-1样基因型.系统进化树结果显示,本研究中国中南部7株G8P[8]分为两大谱系G8-I(13株)和谱系G8-Ⅱ(1株),VP7的最大分支可信度(MCC)树表明,G8的平均进化率估计为1.4×103替换/位点/年.G8P[8]基因型RVA在我国包括两个谱系,从该病毒在中国的出现到该病毒在中国的流行传播将经过1～2年,持续的全国RVA监测至关重要.
Abstract
Rotaviruses of the G8 genotype have emerged and increased rapidly in China.We undertook genome-wide molecular characterization of seven G8P[8]-type RVA strains collected from children under 5 years of age with acute gastroenteritis in south-central China.Rotavirus-specific amplification combined with high-throughput sequencing was used to sequence the whole genome of 14 G8P[8]-type RVA strains from south-central China.Whole-sequence and genetic variation and evolutionary analyses of the RVA strains were carried out using MEGA11.0,Geneious Prime,Geneious9.0.2 and DNASTAR.Seven G8P[8]-type RVA strains were genotyped as G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E1-H2,which was shown to be a DS-1-like genotype.The phylogenetic tree showed that the seven strains of G8P[8]in south-central China were divided into two major lineages:G8-I(13 strains)and lineage G8-Ⅱ(1 strain).The maximum branching confidence(MCC)tree of VP7 indicated that the average evolutionary rate of G8 was estimated to be 1.4 × 10-3 substitutions/locus/year.In summary,the genotype of G8P[8]and RVA comprised two lineages in China.The average evolutionary rate was estimated to be 1.4 × 10-3 substitutions/location/year.The time of emergence to the epidemic spread of the virus in China will take 1-2 years,and continuous national surveillance of RVA is essential.
关键词
轮状病毒/G8P[8]基因型/分子特征/平均进化率Key words
Rotavirus/G8P[8]Genotype/Molecular characterization/Average evolutionary rate引用本文复制引用
基金项目
国家重点研发计划(2022YFC2304302)
国家自然科学基金(21934005)
出版年
2024
NETL
NSTL
万方数据