雾化吸入给药BD-77对呼吸道合胞病毒感染及LPS致小鼠肺炎的治疗作用
Effect of Aerosol Inhalation Administration of BD-77 on Pneumonia Induced by Respiratory Syncytial Virus and Lipopolysaccharide in Mice
孙静 1赵荣华 1孙建辉 1郭姗姗 1高双荣 1包蕾 1耿子涵 1李舒冉 1孙绮悦 1徐洲 2潘玉杰 3陈运琴 3孙田甜 3陈红羽 3黄成钢 2崔晓兰1
作者信息
- 1. 中国中医科学院中药研究所,北京 100700
- 2. 中国科学院上海药物研究所,上海 201203
- 3. 贵州百灵企业集团制药股份有限公司,贵州 561099
- 折叠
摘要
评价BD-77雾化吸入给药对呼吸道合胞病毒(Respiratory syncytial virus,RSV)及脂多糖(Lipopolysaccharide,LPS)致小鼠肺炎的治疗作用,为BD-77的进一步开发提供实验依据.建立RSV及LPS滴鼻感染致小鼠肺炎模型,采用肺指数、肺指数抑制率、病毒载量、肺组织炎症因子等指标评价BD-77的药理作用.BD-77雾化吸入给药75 mg·mL-1、37.5mg·mL-1两个剂量组可显著降低RSV感染小鼠肺指数、病毒载量和炎症因子释放.BD-77静脉注射150 mg/(kg·d)、75 mg/(kg·d)两个剂量组对LPS引起的小鼠肺炎具有保护作用.BD-77对RSV及LPS致小鼠肺炎均具有显著的保护作用.
Abstract
To study the protective effect of BD-77 on pneumonia induced by respiratory syncytial virus(RSV)and lipopolysaccharide(LPS)in mice,and to provide experimental basis for further development of BD-77.A mouse model of pneumonia induced by RSV and LPS was established by nasal drops.The pharmacological effects of BD-77 were evaluated by lung index,lung index inhibition rate,viral load and inflammatory factors of lung tissue.Aerosol administration of BD-77 at 75 mg·mL-1 and 37.5 mg·mL-1 for 25min could significantly reduce lung index,viral load and inflammatory factor release of RSV-infected mice.Intravenous injection of 150 mg/(kg·d)and 75 mg/(kg·d)of BD-77 had protective effects on lipopolysaccharide(LPS)induced pneumonia in mice.BD-77 has significant protective effect on RSV and LPS induced pneumonia in mice.
关键词
BD-77/呼吸道合胞病毒/雾化吸入/肺炎/脂多糖Key words
BD-77/Respiratory syncytial virus/Aerosol inhalation/Pneumonia/Lipopolysaccharide引用本文复制引用
出版年
2024
NETL
NSTL
万方数据