Epidemiological and Genetic Characteristics of Human Bocavirus in Hospitalized Children with Acute Respiratory Tract Infection Cases in Guilin,Guangxi,China
The objective of this study is to delve into the epidemiological and genetic characteristics of Human Boca virus(HBoV)among hospitalized children suffering from acute respiratory infections in Guilin City,Guangxi,China.We employed real-time fluorescence quantitative polymerase chain reaction(qPCR)to detect 15 common respiratory viruses in nasopharyngeal swab samples collected from 638 hospitalized children with acute respiratory infections over the period from September 2021 to October 2022.We conducted nested PCR amplification and sequencing of VP1 gene on HBoV nucleic acid-positive samples.Additionally,we analyzed viral gene characteristics by comparing VP1 sequences of HBoV1 from diverse countries and regions.The results indicated that 50 cases(7.84％)out of 638 acute respiratory infection case samples tested positive for HBoV.Notably,96％(48/50)of HBoV-positive cases were children under the age of 5,with the proportion of children aged 1-2 years being the highest at 40％(20/50).HBoV-positive cases were predominantly observed during summer,autumn and winter.However,no significant difference was observed among these three seasons(P＞0.05).Among the 50 HBoV-positive cases,29 cases(58.00％)were found to be mixed infections.Both single and mixed infections of HBoV can lead to upper or lower respiratory tract infections;however,the difference was not statistically significant(P＞0.05).The 16 HBoV sequences obtained in this study all belonged to HBoV1.There was one sequence distributed in Ia and Ic branches,respectively,while the remaining 14 sequences belonged to Ib branch.HBoV is one of the primary pathogens responsible for respiratory tract infections in children,particularly infants and young children under 2 years old between 2021 and 2022 in the Guilin area,China.Notably,it exhibits common prevalence of three types:Ia,Ib and Ic.Among these,the Ib branch emerged as the dominant strain of HBoV between 2021 and 2022 in Guilin,Guangxi,China.
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