Analysis of Epidemic Trend and Genomic Characteristics of SARS-CoV-2 in Xuzhou,China
In order to understand the epidemic trend of SARS-CoV-2 types in Xuzhou,China and to analyze the molecular characteristics and amino acid mutation differences on the genomic level,a total of 117 samples from local COVID-19 cases in Xuzhou,China from January to May 2023 were selected for whole genome sequencing.The proportion of cases in the"≥80"age group is the highest among sequencing samples,and the overall proportion of male cases is higher than female cases.The age group showed a younger trend.The sequencing results were uploaded to the Pangolin and Nextclade to obtain lineage and mutation information.The sequencing cases were all Omicron variants:with 30 strains of BA.5.2 sub-lineage,58 strains of BF.7 sub-lineage,and 29 strains of XBB sub-lineage.BF.7 sub-lineages were the dominant strains in Xuzhou,China from January to March,while XBB sub-lineages were dominant from April to May.A total of 125 amino acid substitutions were detected in 17 different types of SARS-CoV-2,most of them were located in the spike protein(52).Compared with other lineages,XBB sub-lineages have 3 amino acid substitutions in different directions,12 unique amino acid substitutions and 7 missing amino acid substitutions.The phylogenetic tree showed that BA.5.2 sub-lineages,BF.7 sub-lineages and XBB sub-lineages are divided into three clusters,which is basically consistent with the typing results of Pangolin.Using high-throughput sequencing technology to trace the source and analyze the molecular characteristics of SARS-CoV-2 is of great significance for the study and judgment of the epidemic situation,the establishment of epidemic prevention policies and monitoring of the mutant strains in Xuzhou,China.
SARS-CoV-2OmicronWhole Genome SequencingAmino Acid SubstitutionsPhylogenetic Tree
NETL
NSTL
万方数据
