一叶抗流感胶囊对人冠状病毒229E湿热疫毒袭肺小鼠药理作用研究

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中文摘要：了解一叶抗流感胶囊对人冠状病毒229E(Human coronavirus 229E,HCoV-229E)湿热疫毒袭肺小鼠的药理作用,为扩展其临床应用提供参考.使用HCoV-229E滴鼻感染和湿热环境造模小鼠,考察一叶抗流感胶囊高、中、低不同剂量组[0.704g/(kg·d)、0.352g/(kg·d)、0.176g/(kg·d)三个剂量]对小鼠的肺指数、病毒载量和小鼠肺组织中IL-6、IL-10、TNF-α含量及下丘脑中PGE2、cAMP含量,同时检测小鼠外周血中T淋巴细胞亚群和B淋巴细胞比例,并设西药磷酸氯喹和中成药连花清瘟颗粒作为阳性对照药.一叶抗流感胶囊三个剂量组均可降低小鼠肺指数,高剂量组和中剂量组可降低肺炎模型小鼠的病毒载量,三个剂量组肺组织中TNF-α、IL-6及IL-10含量与模型组比较均下降,下丘脑中cAMP、PGE2含量与模型组比较均降低,且差异具有统计学意义;高、中剂量组外周血CD4+、CD8+T百分比与模型组比较升高,差异有统计学意义;高、中剂量组外周血B细胞百分比与模型组比较均下降,但差异无统计学意义.表明一叶抗流感胶囊对HCoV-229E湿热疫毒袭肺小鼠模型具有治疗作用.

外文标题：Pharmacological Effect of Yiye Anti-influenza Capsule on Dampness-heat Epidemic Virus Pneumonia Mice Infected by HCoV-229E

外文摘要：We investigated the pharmacological effects of Yiye anti-influenza capsule on mice with dampness-heat epidemic virus pneumonia caused by human coronavirus 229E(HCoV-229E)infection to provide a reference for its clinical application.HCoV-229E(nasal drops)was used to infect mice.The Lung Index and viral load,as well as the contents of prostaglandin(PGE)2 and cyclic adenosine monophosphate(cAMP)in the hypothalamus and contents of interleukin(IL)-6,IL-10 and tumor necrosis factor(TNF)-a in the lung tissue of mice were investigated in high-,medium-and low-dose groups of Yiye anti-influenza capsule(0.704 g/(kg·d),0.352 g/(kg·d)and 0.176 g/(kg·d)).The proportion of T-lymphocyte subsets and B lymphocytes in the peripheral blood of mice was detected,and chloroquine phosphate and Lianhua qingwen granules were used as positive controls.The three dose groups of Yiye anti-influenza capsule could reduce the Lung Index of mice.The high-dose group and medium-dose group could reduce the viral load of pneumonia-model mice.The contents of cAMP and PGE2 in the hypothalamus of mice in the three dose groups were decreased compared with those in the model group.The contents of TNF-α,IL-6 and IL-10 in lung tissue were decreased compared with those of the model group,and the difference was significant.The percentage of cluster of differentiation CD4+and CD8+T cells in peripheral blood of high-and medium-dose groups was higher than that of the model group,and the difference was significant.The percentage of B cells did not change significantly compared with that in the model group.Our results showed that Yiye anti-influenza capsule had a therapeutic effect on the mice model of pneumonia caused by HCoV-229E infection.

外文关键词：

Yiye anti-influenza capsuleHuman coronavirus 229ELung IndexViral loadLung tissueHypothalamusPeripheral blood

作者：

张敬升、赵荣华、张薇、冀祖恩、庞博、徐英莉、曹姗、孙静、郭姗姗、彭一峰、崔晓兰

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作者单位：

中国中医科学院中药研究所,北京 100700

新疆甘草及制品研究重点实验室,库尔勒 841011

新疆全安药业股份有限公司,库尔勒 841011

关键词：

一叶抗流感胶囊人冠状病毒229E 肺指数病毒载量肺组织下丘脑外周血

基金：

国家重点研发计划中国中医科学院科技创新工程新疆维吾尔自治区重大科技专项新(疆)地产特色抗病毒新药创制及关键技术研究

项目编号：

2021YFC1712903C12021A046062020A03004

出版年：

2024

DOI：

10.13242/j.cnki.bingduxuebao.004481

病毒学报

中国微生物学会

病毒学报

CSTPCD北大核心

影响因子：1.046

ISSN：1000-8721

年,卷(期)：2024.40(2)

参考文献量31