Preliminary Studies on the Immunogenicity of A Mixed Peptide from the SH Protein of the Human Respiratory Syncytial Virus
The objective of this study was to investigate the immunogenicity and protective effects of the T-cell epitope peptide of human respiratory syncytial virus(HRSV)small hydrophobic protein(SH).Ten peptides covering the full length of the SH protein sequence were designed and synthesized.Using splenic lymphocytes from HRSV-infected mice,five T-cell epitope peptides of SH protein that significantly stimulated interferon-γ,(IFN-γ)production were screened by IFN-γ-Enzyme-Linked Immunospot Assay(ELISpot).The peptides were used to create a mixed peptide vaccine,which was administered subcutaneously twice to C57BL/6J mice along with 5'cytosine-phosphate-guanine 3'oligonucleotide(CpG-OND)and aluminum phosphate(AL)adjuvant.The purpose was to evaluate the vaccine's immunogenicity and protective effect.This study found that the SH protein hybrid peptide vaccine elicited antigen-specific IgG antibodies and T helper 1 cell(Th1)biased T-cell immune responses in immunized mice,compared to the adjuvant control group.Additionally,it reduced the lung viral load and attenuated the pathological damage of the lungs after HRSV infection.These results demonstrate that SH protein peptides can effectively induce immunogenicity and protect mice against HRSV infection.This provides a scientific basis for further research on HRSV peptide vaccines based on SH protein.
Human respiratory syncytial virusSmall hydrophobic proteinPeptideCellular immunity
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