Molecular Identification and Analysis of Capsicum Chlorosis Virus Infecting Tomatoes in Guizhou Province,China
Capsicum chlorosis virus(CaCV)is an important member of the Orthotospovirus genus.CaCV infects chili peppers and tomatoes and causes serious harm.We wished to clarify the molecular structural characteristics and evolutionary origin of the genome of CaCV isolates in tomatoes from Guizhou Province,China.We cloned full-length fragments of the N,NSs,and NSm genes of CaCV using real-time reverse transcription-polymerase chain reaction.We obtained target bands of approximately 828 bp,927 bp,and 1320 bp in length,respectively.The sequence alignment showed that the N,NSs,and NSm genes of CaCV isolates in tomatoes from Guizhou,China,had nucleotide similarities with each CaCV isolate,which ranged from 85.5％to 99.4％,82.7％to 98.9％,and 83.1％to 98.7％,respectively.According to phylogenetic trees,the N gene and NSs gene of CaCV isolates in tomatoes from Guizhou,China were closely related to those of CaCV isolates in tomatoes from Yunnan,China,whereas the NSm gene was closely related to CaCV isolates in tomatoes from Hainan Province and Guangzhou Province in China.We speculated that CaCV in Guizhou may have been due to the:(i)introduction of tomato and chili varieties;(ii)transmission of thrips carrying viruses.This is the first report of CaCV infection in tomatoes in Guizhou,China,which is important for monitoring the spread of CaCV in China.
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