Identification of the T-cell Epitope in BALB/c Mice Immunized with Adenovirus Vector EBV Vaccine Expressing gH/gL Antigen
We wished to identify the T-cell epitopes of an adenovirus vector vaccine expressing the protective antigen of gH/gL of the Epstein-Barr virus(EBV)in BALB/c mice.We immunized BALB/c mice with an adenovirus vector EBV vaccine expressing gH/gL protein.We predicted the affinity of the sequence with Immune Epitope Database Analysis Resource software.Next,we designed and synthesized the peptide library of gH and gL of EBV,and mixed them into a"peptide pool".Interferon(IFN)-γ in immunized mouse spleen cells was detected using enzyme-linked immunosorbent spot(ELISpot).Peptide fragments with a positive reaction were detected by two rounds of peptide-pool and single-peptide screening to determine the dominant reactive peptide.Five specific dominant peptides of gH were screened out,among which the single peptide with the sequence of LRGPFSYPSLTSAQS had the strongest reaction.There were four specific dominant peptides in gL,among which the single peptide with the sequence ASLNSPKNGSNQLVI had the strongest reaction.In summary,an ELISpot method based on IFN-γ was used to screen and determine nine dominant T-cell epitopes specific to the gH antigen and gL antigen of EBV.Our method provides a reference for EBV immunology and vaccine research.
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