摘要
随着人们对病毒抗原结构的深入认识,基于蛋白质结构的抗原设计已经成为新型疫苗研发的重要手段.本文总结了以结构生物学为基础的结构疫苗学近年来的发展进程及研究方向,重点介绍了基于氨基酸位点突变的结构疫苗学设计方法,包括二硫键取代、脯氨酸取代、空腔疏水取代等,并讨论这些方法在人类免疫缺陷病毒、呼吸道合胞病毒、人偏肺病毒、新冠病毒、流感病毒及人乳头瘤病毒等病毒疫苗研发中的具体应用.
Abstract
Thanks to deepening understanding of the structure of virus antigens,structure-based antigen design has become an important means of developing new vaccines.Herein,we summarize the recent developments and research directions of structural vaccinology based on structural biology.We focus on the design method of structural vaccinology based on mutations of amino-acid sites,including the substitution of disulfide bonds,proline substitution,and cavity hydrophobic substitution.We also discuss the specific applications of these methods in the development of vaccines against the human immunodeficiency virus,respiratory syncytial virus,human metapneumovirus,severe acute respiratory syndrome-coronavirus-2,influenza virus,and human papillomavirus.
基金项目
昆明市国际(对外)科技合作基地项目(GHJD-2021016)
云南省科技重大专项(202202AA 100014)
NETL
NSTL
万方数据