Mechanism of Action of Montelukast Sodium Against Pulmonary Fibrosis in Mice with Bronchial Asthma
Intercellular adhesion molecule(ICAM)-1 is one of the receptors mediating human rhinovirus(HRV)infection in bronchial asthma(BA).It plays a key part in the regulation of airway reactivity,lung and bronchial injury,and inflammatory factors.Montelukast can alleviate airway reactivity,reduce damage to the lung and trachea,and has anti-inflammatory effects.Whether montelukast sodium(MS)downregulates ICAM-1 expression to prevent BA caused by HRV infection is.not known.In this study,BALB/c mice were divided randomly into four groups of 30:normal control,normal treatment,model control,and model treatment.BA models were constructed by RV-1B in the model control group(MCG)and model treatment group(MTG),and mice in the MTG received MS.Mice in the normal control group(NCG)and normal treatment group(NTG)were normal mice,and mice in the NTG received MS.The copy number of RV-1B in the lung tissues of each group of mice was determined.The level of immunoglobulin(Ig)E,tumor necrosis factor(TNF)-α,interleukin(IL)-4,IL-13,cysteinyl leukotriene receptor type 1 antibody(CysLT1)and interferon(IFN)-γ in the bronchoalveolar lavage fluid(BALF)of mice from each group was determined.Pathological changes in each group were observed.Protein and mRNA expression of ICAM-1 as well as the apoptosis level of eosinophils(EOS)in the lung tissues of each group were determined.Excessive deposition of collagen fibers in alveolar septa and bronchus as well as red cells oozing out of the alveolar cavity were observed in the MCG.Compared with the MCG,decreased deposition of collagen fibers in alveolar septa and bronchus as well as fewer red cells oozing out of the alveolar cavity were observed in the MTG.The copy number of RV-1B increased and then decreased in the MCG and MTG,but there was no significant change in the copy number of RV-1B at each time point in either group.Compared with that of the MCG,mRNA and protein expression of ICAM-1 decreased obviously and the apoptosis index for EOS increased significantly in the MTG.Compared with that of the MCG,the IFN-γ level in BALF increased,but the level of IgE,TNF-α,IL-4,IL-13 and CysLT1 in BALF decreased in the MTG.Therefore,MS could alleviate the pulmonary fibrosis caused by RV-1B infection by promoting EOS apoptosis and reducing inflammation via downregulating expression of the RV-1B receptor target ICAM-1.
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