Hepatitis B Virus Promotes the Proliferation and Migration of Liver Cancer Cells Through the MiR-154-5p/STAT3 Axis
To investigate the effect of hepatitis B virus(HBV)on the proliferation and migration of liver cancer cells through the miR-154-5p/STAT3 axis.HepG2 cells were infected with adenovirus containing 1.3-fold overlength HBV genome,and normal HepG2 was used as control(NC),which was denoted as HBV group and NC group.Quantitative real-time PCR(qRT-PCR)was used to detect the expression level of miR-154-5p in HBV group,NC group,human hepatocellular carcinoma cell HepG2.2.15 with stable HBV expression and human hepatocellular carcinoma cell HepG2.Transfected miR-NC mimic,overexpression miR-154-5p,overexpression miR-154-5p+empty vector pcDNA,overexpression miR-154-5p+overexpression STAT3 into HepG2.2.15 cells according to the liposome method,denoted as miR-NC group,miR-154-5p group,miR-154-5p+pcDNA group,miR-154-5p+STAT3 group.Cell counting kit(CCK8),clone formation test to detect cell proliferation;wound healing test to detect cell migration;Western blot method to detect signal transduction and transcription factor 3(STAT3),proliferating cell nuclear antigen(PCNA),matrix metalloproteinase 2(MMP2),matrix metalloproteinase 9(MMP9)protein expression.The luciferase experiment detects the relationship between miR-154-5p and STAT3.Compared with the NC group,the expression level of miR-154-5p in the HBV group was reduced;compared with the HepG2 cells,the expression level of miR-154-5p in the HepG2.2.15 cell was significantly reduced.Up-regulation of miR-154-5p significantly reduced cell viability,number of clones,migration rate,and inhibited PCNA,MMP2,and MMP9 protein expression.miR-154-5p targets and negatively regulates the expression of STAT3.Overexpression of STAT3 can reverse the inhibiting effect of miR-154-5p up-regulation on the proliferation and migration of HepG2.2.15 cells.HBV inhibits miR-154-5p expression to upregulate STAT3,thereby promoting the proliferation and migration of liver cancer cells.
Hepatitis B virusMiR-154-5pSTAT3Liver cancer cellsProliferationMigration
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