Phylogenetic Analysis of Influenza A/H3N2 in Binzhou,China During 2012-2023
To analyze the genetic and antigenic characterization of influenza A/H3N2 virus in Binzhou,China during 2012-2023.Throat swabs were collected from influenza-like illness(ILI)from 2012 to 2023 in Binzhou,China.Virus isolation was performed.45 representative influenza A/H3N2 isolates were selected for whole genome sequencing.Phylogenetic analysis of whole-genome sequences was performed using MegAlign and MEGA software.A total of 10137 samples were obtained and the overall positive rate of influenza virus(A/H3N2,A/H1N1,BY、BV)was 16.2％.Our surveillance data indicated that Influenza A/H3N2 virus was detected in eight years,the positive rates were 9.6％,1.6％,6.0％,0.1％,10.4％,7.3％,19.0％and 8.8％,respectively.Statistically significant differences between the positive rate of influenza A/H3N2 viruses were observed(x2=775.902,P＜0.001).The median pairwise sequence identities were ranging from 97.2％～99.0％for 8 segments of 45 virus sequences.Segment of all A/H3N2 viruses analyzed belonged to the corresponding subclade,reassortment was not observed.There were revert mutations at 128(A-T-A-T),131(T-K-T-K),135(T-K-T),138(A-S-A)sites on HA gene.The whole-genome sequence of influenza A/H3N2 virus detected three evolvement,which were located in four main genetic clades during 2012-2023.The mutation sites were located at antigenic determinant positions of HA gene which caused the genetic clade evolution.Antigenic drift variant is the basis of genetic clade evolution of influenza viruses.
Influenza A H3N2 virusWhole genome sequenceHomologyClade evolution
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