Analysis of Occult Hepatitis-B Infection in Non-discriminating Reactive Donors Based on the Nucleic-acid Test
We wished to explore occult hepatitis-B infection(OBI)in non-discriminating reactive donors(NDRDs)based on the nucleic-acid test in Shaoxing,China.A chemoluminescence test was used to detect hepatitis B surface antigen(HBsAg).The nucleic-acid test was used to detect hepatitis-B virus(HBV)DNA.A chemiluminescence test was employed to detect hepatitis B surface antibody(HBsAb)for the first tracking test.Simultaneously,76 samples of blood from donors negative for HBsAg and HBV DNA were selected.Fifty-two samples of blood from donors confirmed as having OBI were selected as the control to detect HBsAb.Blood donors negative for HBsAg and positive for HBV DNA were determined to have OBI.Statistical analyses were conducted on the proportion of HBsAb that was positive in different populations.Statistical analyses were conducted on NDRDs confirmed as having OBI based on sex,age,educational background,HBsAb concentration in serum,and frequency of the tracking test.Sixty-six individuals were tracked and tested,of which 12 were confirmed to have OBI.The proportion of OBI in NDRDs was 18.18％.There was no significant difference in the proportion of blood donors confirmed as having OBI according to sex and educational background(P＞0.05).There was a significant difference in the proportion of blood donors confirmed as having OBI in different ages(P＜0.05).There was no significant difference in the proportion of HBsAb that was positive among blood donors who were negative for HBsAg and HBV DNA,NDRDs,and those confirmed as having OBI(P＞0.05).There was a significant difference in the proportion of blood donors confirmed as having OBI in different HBsAb concentrations in serum in NDRDs(P＜0.05).There was a significant difference in the proportion of OBI detection in different tracking tests(P＜0.05).The proportion of OBI in NDRDs was high.The older the NDRD,the higher the proportion of OBI.OBI was not found in NDRDs with a HBsAb concentration in serum＞300 IU/L.A HBsAb concentration in serum＞300 IU/L could be used as an indicator for the retesting of NDRDs.The proportion of OBI confirmed in NDRDs after ≥5 follow-up tests was significantly high.To increase the detection rate of OBI in NDRDs,the number of nucleic-acid tests can be increased to ≥5.
Non-discriminating reactive donorsBlood donorNucleic-acid testOccult hepatitis-B infectionHBsAbNumber of detections
万方数据
维普
