Preliminary Study on Preparation and Immunogenicity of SARS-CoV-2 Subunit Vaccine
Since the widespread of SARS-CoV-2 in the world at the end of 2019,it has had a huge negative impact on human society.In this paper,we reported a tandem subunit nanoparticle vaccine based on the sequence of the receptor binding domain(RBD)of the SARS-CoV-2 mutant strain XBB1.5 as the core molecule of the vaccine and concatenating the full-length S protein of SARS-CoV-2.In order to improve the expression level and the stability of the protein molecule,mutations of six proline residues were added.Moreover,modification of the cleavage site of Flynase was added to increase stability.Then,the amino acid sequence of human ferritin was connected,and the characteristics of multiple antigen proteins were combined on the surface of ferritin nanoparticles to increase the stimulation of antigen epitopes and increase antigen presentation.After immunizing mice with the combination of aluminum hydroxide and CpG1018 double adjuvant,the mice were produced neutralizing antibodies against multiple variants of COVID-19,and triggered relatively strong cellular and humoral immunity.Overall,the data in this paper suggest that the XBB1.5 mutant RBD plus S protein full-length tandem ferritin nanoparticle vaccine is effective against the SARS-CoV-2 variant and may have the ability to prevent future Sarbecvirus variants.
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