Detection of Herpesvirus from Tissue Cells of Cultured Largemouth Bass(Micropterus salmoides)with Disease
Herpesvirus was first detected from diseased cultured largemouth bass(Micropterus salmoides)by the ultrastructual pathological study and PCR detection.Herpesvirus was detected from the cell of gill and liver of the diseased Largemouth bass by electron microscope examination.Virions in gill epithelial cells,intestinal epithelial and liver cells were round,double-shell in shape or hollow and about 70 nm to 80 nm in diameter.Around the nucleus of the liver cell there were envelopes with diameter about 100 nm.The morphological and morphogenetic characteristics of the virus detected from the cultured Largemouth bass are comparable with the morphology of the family of Herpesviridae.We investigated in 9 farms,24 samples of diseased cultured Largemouth bass were detected by PCR,13 samples were positive for herpesvirus.Based on the sequence of DNA polymerase gene fragment of the herpesvirus strain of largemouth bass,the phylogenetic tree analysis further proves that the virus may be a member of the genus Cyprinus herpesvirus.
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