首页|Development of a High-throughput Sequencing Platform for Detection of Viral Encephalitis Pathogens Based on Amplicon Sequencing

Development of a High-throughput Sequencing Platform for Detection of Viral Encephalitis Pathogens Based on Amplicon Sequencing

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Objective Viral encephalitis is an infectious disease severely affecting human health.It is caused by a wide variety of viral pathogens,including herpes viruses,flaviviruses,enteroviruses,and other viruses.The laboratory diagnosis of viral encephalitis is a worldwide challenge.Recently,high-throughput sequencing technology has provided new tools for diagnosing central nervous system infections.Thus,In this study,we established a multipathogen detection platform for viral encephalitis based on amplicon sequencing.Methods We designed nine pairs of specific polymerase chain reaction(PCR)primers for the 12 viruses by reviewing the relevant literature.The detection ability of the primers was verified by software simulation and the detection of known positive samples.Amplicon sequencing was used to validate the samples,and consistency was compared with Sanger sequencing.Results The results showed that the target sequences of various pathogens were obtained at a coverage depth level greater than 20×,and the sequence lengths were consistent with the sizes of the predicted amplicons.The sequences were verified using the National Center for Biotechnology Information BLAST,and all results were consistent with the results of Sanger sequencing.Conclusion Amplicon-based high-throughput sequencing technology is feasible as a supplementary method for the pathogenic detection of viral encephalitis.It is also a useful tool for the high-volume screening of clinical samples.

Viral encephalitisAmplicon sequencingHigh-throughput sequencingMultipathogen detection

ZHANG Ya Li、SU Wen Zhe、WANG Rui Chen、LI Yan、ZHANG Jun Feng、LIU Sheng Hui、HU Dan He、XU Chong Xiao、YIN Jia Yu、YIN Qi Kai、HE Ying、LI Fan、FU Shi Hong、NIE Kai、LIANG Guo Dong、TAO Yong、XU Song Tao、MA Chao Feng、WANG Huan Yu

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Shaanxi University of Chinese Medicine,Xianyang 712000,Shaanxi,China

National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China

Guangzhou Center for Disease Control and Prevention,Guangzhou 510000,Guangdong,China

Shandong Center for Disease Control and Prevention,Jinan 250000,Shandong,China

Department of Ophthalmology,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China

Xi'an Center for Disease Control and Prevention,Xi'an 710054,Shaanxi,China

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国家重点研发计划

2022YFC2305304

2024

生物医学与环境科学(英文版)
中国疾病预防控制中心

生物医学与环境科学(英文版)

CSTPCD
影响因子:0.76
ISSN:0895-3988
年,卷(期):2024.37(3)
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