首页|Salidroside Ameliorates Lung Injury Induced by PM2.5 by Regulating SIRT1-PGC-1α in Mice

Salidroside Ameliorates Lung Injury Induced by PM2.5 by Regulating SIRT1-PGC-1α in Mice

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Objective This study aimed to clarify the intervention effect of salidroside(SAL)on lung injury caused by PM2.5 in mice and illuminate the function of SIRT1-PGC-1ɑ axis.Methods Specific pathogen-free(SPF)grade male C57BL/6 mice were randomly assigned to the following groups:control group,SAL group,PM2.5 group,SAL+PM2.5 group.On the first day,SAL was given by gavage,and on the second day,PM2.5 suspension was given by intratracheal instillation.The whole experiment consist of a total of 10 cycles,lasting 20 days.At the end of treatment,blood samples and lung tissues were collected and analyzed.Observation of pathological changes in lung tissue using inverted microscopy and transmission electron microscopy.The expression of inflammatory,antioxidants,apoptosis,and SIRT1-PGC-1ɑ proteins were detected by Western blotting.Results Exposure to PM2.5 leads to obvious morphological and pathologica changes in the lung of mice.PM2.5 caused a decline in levels of antioxidant-related enzymes and protein expressions of HO-1,Nrf2,SOD2,SIRT1 and PGC-1ɑ,and an increase in the protein expressions of IL-6,IL-1β,Bax,caspase-9 and cleaved caspase-3.However,SAL reversed the aforementioned changes caused by PM2.5 by activating the SIRT1-PGC-1α pathway.Conclusion SAL can activate SIRT1-PGC-1ɑ to ameliorate PM2.5-induced lung injury.

PM2.5SalidrosideOxidative stressInflammationApoptosisSIRT1-PGC-1α

LI Xiao Hong、LIU Yu Mei、SHAN Hui、TAN Jin Feng、ZHOU Jian、SONG Yuan Jin、LI Si Qi、LIU Chen、XU Dong Qun、YU Li、LI Wan Wei

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School of Public Health,Shandong Second Medical University,Weifang 261053,Shandong,China

Key Laboratory of Health Inspection and Quarantine,Weifang 261053,Shandong,China

Weifang Environmental Monitoring Station,Weifang 261042,Shandong,China

Department of Orthopedics,The 80th Army Hospital of PLA,Weifang 261000,Shandong,China

Environment and Health Related Product Safety Institute of Chinese Center for Disease Control and Prevention,Beijing 100021,China

School of Basic Medicine,Shandong Second Medical University,Weifang 261053,Shandong,China

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Shandong Provincial Natural Science Foundation,ChinaWeifang Science and Technology Development Plan ProjectWeifang Science and Technology Development Plan Project

ZR2020MH3362022GX0152022GX010

2024

10.3967/bes2024.041

生物医学与环境科学(英文版)
中国疾病预防控制中心

生物医学与环境科学(英文版)

CSTPCD
影响因子：0.76
ISSN：0895-3988
年,卷(期)：2024.37(4)
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