首页|Changes in the Non-targeted Metabolomic Profile of Three-year-old Toddlers with Elevated Exposure to Polycyclic Aromatic Hydrocarbons

Changes in the Non-targeted Metabolomic Profile of Three-year-old Toddlers with Elevated Exposure to Polycyclic Aromatic Hydrocarbons

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Objective To investigate changes in the urinary metabolite profiles of children exposed to polycyclic aromatic hydrocarbons(PAHs)during critical brain development and explore their potential link with the intestinal microbiota.Methods Liquid chromatography-tandem mass spectrometry was used to determine ten hydroxyl metabolites of PAHs(OH-PAHs)in 36-month-old children.Subsequently,37 children were categorized into low-and high-exposure groups based on the sum of the ten OH-PAHs.Ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to identify non-targeted metabolites in the urine samples.Furthermore,fecal flora abundance was assessed by 16S rRNA gene sequencing using Illumina MiSeq.Results The concentrations of 21 metabolites were significantly higher in the high exposure group than in the low exposure group(variable importance for projection>1,P<0.05).Most of these metabolites were positively correlated with the hydroxyl metabolites of naphthalene,fluorine,and phenanthrene(r=0.336-0.531).The identified differential metabolites primarily belonged to pathways associated with inflammation or proinflammatory states,including amino acid,lipid,and nucleotide metabolism.Additionally,these distinct metabolites were significantly associated with specific intestinal flora abundances(r=0.34-0.55),which were mainly involved in neurodevelopment.Conclusion Higher PAH exposure in young children affected metabolic homeostasis,particularly that of certain gut microbiota-derived metabolites.Further investigation is needed to explore the potential influence of PAHs on the gut microbiota and their possible association with neurodevelopmental outcomes.

ChildGut microbiotaNon-targeted metabolomicsPolycyclic aromatic hydrocarbonsUrinary metabolite profile

LI Yang、LIN Dan、ZHANG Xiu Qin、JU Guang Xiu、SU Ya、ZHANG Qian、DUAN Hai Ping、YU Wei Sen、WANG Bing Ling、PANG Shu Tao

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Department of Epidemiology and Health Statistics,School of Public Health,Qingdao University,Qingdao 266021,Shandong,China

Department of Environmental Health,Qingdao Municipal Center for Disease Control and Prevention,Qingdao Institute of Preventive Medicine,Qingdao 266033,Shandong,China

Physicochemical Laboratory,Qingdao Municipal Center for Disease Control and Prevention,Qingdao Institute of Preventive Medicine,Qingdao 266033,Shandong,China

Section of Child Health Care,Huangdao Maternity and Child Health Care Hospital of Qingdao,Qingdao 266499,Shandong,China

Qingdao Blood Center,Qingdao 266071,Shandong,China

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国家自然科学基金BiosTime Maternal and Infant Nutrition and Health Research FundProjects of Medical and Health Technology Development Program in Shandong ProvinceQingdao Outstanding Health Professional Development Fund(,)(2020-2022、2022-2024)

813729552017FYH0082016WS0309

2024

10.3967/bes2024.053

生物医学与环境科学(英文版)
中国疾病预防控制中心

生物医学与环境科学(英文版)

CSTPCD
影响因子：0.76
ISSN：0895-3988
年,卷(期)：2024.37(5)