DNA Methylation of KLRC1 and KLRC3 in Autoimmune Thyroiditis:Perspective of Different Water Iodine Exposure

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外文摘要：Objective This study aimed to identify differentially methylated genes (DMGs) associated with natural killer cells in patients with autoimmune thyroiditis (AIT),focusing on the influence of varying water iodine exposure levels.Methods Participants were divided into categories based on median water iodine (MWI) concentrations:iodine-fortified areas (IFA,MWI<10 μg/L),iodine-adequate areas (IAA,40 ≤ MWI ≤ 100μg/L),and iodine-excessive areas (IEA,MWI>300 μg/L). A total of 176 matched AIT cases and controls were recruited and divided into 89,40,and 47 pairs for IFA,IAA,and IEA,respectively. DMGs were identified using 850K BeadChip analysis for 10/10 paired samples. Validation of DNA methylation and mRNA expression levels of the DMGs was conducted using MethylTarget™ and QRT-PCR for 176/176 paired samples.Results KLRC1,KLRC3,and SH2D1B were identified as significant DMGs. Validation revealed that KLRC1 was hypomethylated and highly expressed,whereas KLRC3 was hypermethylated and highly expressed in individuals with AIT. Furthermore,KLRC1 was hypomethylated and highly expressed in both IFA and IEA.Conclusion The DNA methylation status of KLRC1 and KLRC3 may play crucial roles in AIT pathogenesis. Additionally,DNA methylation of KLRC1 seems to be influenced by different iodine concentrations in water.

外文关键词：

Autoimmune thyroiditisDNA methylationKLRC1KLRC3SH2D1BIodine

作者：

Yao Chen、Jinjin Liu、Mengying Qu、Bingxuan Ren、Huaiyong Wu、Li Zhang、Zheng Zhou、Lixiang Liu、Hongmei Shen

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作者单位：

Disorders Control,Centre for Endemic Disease Control,Chinese Centre for Disease Control and Prevention,Harbin Medical University,Harbin 150081,Heilongjiang,China

National Health Commission & Education Bureau of Heilongjiang Province,Key Laboratory of Etiology and Epidemiology,Harbin Medical University,Harbin 150081,Heilongjiang,China

Heilongjiang Provincial Key Laboratory of Trace Elements and Human Health,Harbin Medical University,Harbin 150081,Heilongjiang,China

Division of Health Risk Factor Monitoring and Control,Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China

Department of Public Health Administration,Affiliated Hospital of Jining Medical University,Jining 272060,Shandong,China

Heze Municipal Hospital,Shandong Provincial Hospital Heze Hospital,Heze 274700,Shandong,China

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基金：

National Natural Science Foundation of China

项目编号：

82073490

出版年：

2024

DOI：

10.3967/bes2024.103

生物医学与环境科学(英文版)

中国疾病预防控制中心

生物医学与环境科学(英文版)

CSTPCD

影响因子：0.76

ISSN：0895-3988

年,卷(期)：2024.37(9)