首页|blaNDM-1 Carried by a Transferable Plasmid in a Salmonella Strain Isolated from Healthy Individuals

blaNDM-1 Carried by a Transferable Plasmid in a Salmonella Strain Isolated from Healthy Individuals

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Objective Our study aimed to conduct genomic characterization of Salmonella strains carrying the b/aNDM-1 gene in the intestinal tract of healthy individuals.The objectives were to underscore the importance of genomic surveillance for drug resistance in both commensal and pathogenic bacteria among healthy populations,and to establish protocols for regulating drug resistance plasmids based on the completion of a comprehensive map of drug resistance plasmid genomes.Methods We performed antimicrobial susceptibility testing and employed second-and third-generation sequencing techniques to analyze Salmonella strains harboring the b/aNDM-1 gene,to surveil drug-resistant bacteria in the intestines of healthy subjects.Sequence comparison was conducted using both core-and pan-genome approaches.Concurrently,conjugation experiments were carried out to assess the efficiency of plasmid transfer.Results We isolated a carbapenem-resistant Salmonella enterica serovar Typhimurium strain from a healthy food worker in China.This strain harbored an lncHI2/lncHI2A plasmid carrying blaNDM-1 along with multiple antibiotic resistance genes(ARGs).Our findings highlight the potential for asymptomatic carriers to facilitate the transmission of ARGs.Pan-genomic analysis revealed that b/aNDM-1-positive plasmids could traverse bacterial species barriers,facilitating cross-host transmission.Conclusion This study marks the first detection of b/aNDM-1 in Salmonella strains isolated from healthy individuals.We underscore the risk associated with the transmission of conjugative hybrid plasmids carrying blaNDM-1,which have the potential to be harbored and transmitted among healthy individuals.Enhanced surveillance of drug-resistant pathogens and plasmids in the intestinal microbiota of healthy individuals could provide insights into the risk of ARG transmission and pathways for population-wide dissemination via ARG transfer factors.

CarbapenemsNDM-1lncHI2/lncHI2A plasmidSalmonella TyphimuriumHealthy individual

Wei Zeng、Ming Luo、Pengcheng Du、Zhenpeng Li、Yao Peng、Mengyu Wang、Wenxuan Zhao、Huayao Zhang、Yang Li、Pengjie Luo、Yannong Wu、Jialiang Xu、Xu Li、Xin Lu、Biao Kan

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Yantai Center for Disease Control and Prevention,Yantai 264003,Shandong,China

National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China

School of Public Health,Shandong University,Jinan 250012,Shandong,China

Yulin Center for Disease Control and Prevention,Yulin 537000,Guangxi,China

Qitan Technology Ltd.,Chengdu 610044,Sichuan,China

NHC Key Laboratory of Food Safety Risk Assessment,China National Center for Food Safety Risk Assessment(CFSA),Beijing 100022,China

School of Food and Chemical Engineering,Beijing Technology and Business University,Beijing 100048,China

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2024

10.3967/bes2024.104

生物医学与环境科学(英文版)
中国疾病预防控制中心

生物医学与环境科学(英文版)

CSTPCD
影响因子：0.76
ISSN：0895-3988
年,卷(期)：2024.37(11)