This experiment was designed to measure the activity of glutathione S-transferase(GSTs)in diverse tissues of the fifth-instar larvae of both parental and F1 generations of Antheraea pernyi.Through the utilization of biostatistical methodologies,the correlation between GSTs activity and eco-nomic traits was meticulously analyzed,with the objective of probing into the potential of employing GSTs activity to predict the hybrid vigor in Antheraea pernyi.The results manifest that disparities exist in the hybrid vigor of GSTs activity among different tissues of Antheraea pernyi larvae.The correlation between GSTs activity in diverse tissues and economic traits exhibits variation.Specifical-ly,in the silk gland,GSTs activity demonstrates an extremely significant positive correlation with the cocooning rate and also an extremely significant positive correlation with the heterosis rate.In the fat body and silk gland,GSTs activity displays a significant positive correlation with the number of co-coons harvested per single moth,and it is also significantly positively correlated with the correspond-ing heterosis rate.Therefore,GSTs activity can act as an auxiliary candidate biochemical index for predicting the hybrid vigor of yield traits.