Based on the method of incubation test by contrasting the urea of without nitrification inhibitor added, this paper compared the effect of DMPP and its isomers 3,5- Dimethylpyrazole phosphate as nitrification inhibitors on the transform of urea nitrogen in soil. The results showed that 3,5 - Dimethylpyrazole phosphate has definite role of nitrification inhibition. During the period of cultivation, a week later the content of soil nitrate nitrogen by adding 3,5- Dim - ethylpyrazole phosphate is all along lower than that of contrast, and the content of soil ammonium nitrogen is all along higher than that of contrast. But in the later period (three weeks later), the nitrification inhibiting effect of 3, 5 - Dimethylpyrazole phosphate as a nitrification inhibitor is inferior than DMPP. 3,5- Dimethypyrazole phosphate can or not become a new nitrification inhibitor, we should do more research on its efficiency of physiology and ecology.