北方药学2024,Vol.21Issue(1) :5-8.DOI:10.3969/j.issn.1672-8351.2024.01.002

灯芪通脉颗粒高效液相色谱法指纹图谱研究

Study of High Performance Liquid Chromatography Fingerprint of Deng-Qi-Tong-Mai Granule

台晶杰 甄会贤 张凤 王小露 张虹
北方药学2024,Vol.21Issue(1) :5-8.DOI:10.3969/j.issn.1672-8351.2024.01.002

灯芪通脉颗粒高效液相色谱法指纹图谱研究

Study of High Performance Liquid Chromatography Fingerprint of Deng-Qi-Tong-Mai Granule

台晶杰 1甄会贤 1张凤 1王小露 1张虹1
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作者信息

  • 1. 山西药科职业学院,山西 太原 030031
  • 折叠

摘要

目的:建立灯芪通脉颗粒的指纹图谱法,指认共有成分并评价质量.方法:使用 Diamonsil C18色谱柱(4.6 mm×250 mm,5 μm),流动相为水(含 0.1%磷酸)-乙腈,梯度洗脱,检测波长为 230nm.对 10 批灯芪通脉颗粒指纹图谱分析,并建立共有模式计算样品间的相似度.结果:样品具有较高的相似性.结论:本方法可用于评价灯芪通脉颗粒质量.

Abstract

Objective:To establish a fingerprinting method of Deng-Qi-Tong-Mai Granule for identifying common components and evaluating the quality.Method:The Diamonsil C18 chromatographic column(4.6 mm×250 mm,5 μm)was used with a mobile phase consisting of water(containing 0.1%phosphoric acid)and acetonitrile in a gradient elution mode,with a detection wavelength of 230 nm.Fingerprint analysis was conducted on ten batches of Deng-Qi-Tong-Mai Granule,and a common pattern was established to calculate the similarity between the samples.Results:The samples exhibited high similarity.Conclusion:This fingerprinting method can be used to evaluate the quality of Deng-Qi-Tong-Mai Granule.

关键词

指纹图谱/高效液相色谱/灯芪通脉颗粒/质量评价

Key words

fingerprint/high performance liquid chromatography/Deng-Qi-Tong-Mai Granule/quality evaluation

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出版年

2024
北方药学
内蒙古自治区食品药品学会

北方药学

影响因子:0.633
ISSN:1672-8351
参考文献量11
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