Study of High Performance Liquid Chromatography Fingerprint of Deng-Qi-Tong-Mai Granule
Objective:To establish a fingerprinting method of Deng-Qi-Tong-Mai Granule for identifying common components and evaluating the quality.Method:The Diamonsil C18 chromatographic column(4.6 mm×250 mm,5 μm)was used with a mobile phase consisting of water(containing 0.1%phosphoric acid)and acetonitrile in a gradient elution mode,with a detection wavelength of 230 nm.Fingerprint analysis was conducted on ten batches of Deng-Qi-Tong-Mai Granule,and a common pattern was established to calculate the similarity between the samples.Results:The samples exhibited high similarity.Conclusion:This fingerprinting method can be used to evaluate the quality of Deng-Qi-Tong-Mai Granule.