Objective:To establish a qualitative and quantitative evaluation method for the medicinal materials of hollyhock.Methods:The qualitative identification method of hollyhock flower was established by microscopy and thin layer chromatography,The 2020 edition of the"Chinese Pharmacopoeia"method for the determination of moisture,total ash,acid-insoluble ash,and extract content,and the quantitative analysis method of kaempferol in hollyhock flower was established by HPLC.Results:The established TLC had clear spots and good repeatability,which could characteristically realize the qualitative identification of 7 batches of hollyhock.Measured the moisture,total ash,acid-insoluble ash,and extract content of 7 batches of samples.The standard curve for the determination of kaempferol in hollyhock is Y=0.0471X-0.0288,the linear range is 10.81ng～135.13 ng,the correlation coefficient is 0.9999,the precision RSD=0.87%,the average sample recovery rate is 102.03%,RSD=1.82%.The content of kaempferol in 7 batches of hollyhock flowers was 0.12%～0.49%.Conclusion:The established microscopic method and thin-layer chromatography can realize the qualitative identification of hollyhock medicinal materials.High performance liquid chromatography is simple,feasible,and can be used for the content determination of kaempferol in hollyhock.
hollyhock floskaempferolmicroscopic identificationthin layer chromatographHigh-performance liquid chromatographystudy on quality standard
NETL
NSTL
万方数据
