酶辅助提取盐肤木总三萜工艺研究
Study on Enzymatic Assisted Extraction of Total Triterpenes from Rhus chinensis
贾雯 1林瑶 1徐惠龙 1许文 1徐伟1
作者信息
- 1. 福建中医药大学药学院,福建福州 350122
- 折叠
摘要
目的:确定酶辅助提取盐肤木三萜类成分的工艺参数.方法:采用紫外分光光度法测定盐肤木总三萜含量.以总三萜含量为评价指标,通过单因素试验筛选酶辅助提取的主要影响因素及水平,进行响应面优化.结果:酶辅助提取的最优条件为:添加 2000 U(40 mg)纤维素酶,调节p H5.4,45℃下酶解75 min.在最佳酶辅助提取条件下进行验证试验,盐肤木中三萜化合物的提取得率为1.186%,与预测值接近.结论:该工艺合理可行,可为盐肤木三萜类成分提取研究提供参考.
Abstract
Objective:To determine the process parameters for enzyme assisted extraction of triterpenoid components from Rhus chinensis.Method:The total triterpenoid content of Rhus chinensis was determined by UV spectrophotometry.Using the total triterpenoid content as the evaluation index,the main influencing factors and levels of enzyme assisted extraction were screened through single factor experiments,and response surface optimization was carried out.Result:The optimal conditions for enzyme assisted extraction were:adding 2000 U(40 mg)of cellulase,adjusting pH 5.4,and enzymatic hydrolysis at 45℃for 75 minutes.Under the optimal enzyme assisted extraction conditions,a validation experiment was conducted,and the extraction yield of triterpenoids in Rhus chinensis was 1.186%,which was close to the predicted value.Conclusion:This process is reasonable and feasible,and can provide a reference for the extraction of triterpenoid components from Rhus chinensis.
关键词
盐肤木/总三萜/提取/响应面法/酶添加量/pH/温度/时间Key words
Rhus chinensis/total triterpenes/extract/response surface methodology/enzyme addition amount/pH/temperature/time引用本文复制引用
出版年
2024
NETL
NSTL
万方数据