Study on Quality Control of Shenhuang Detoxification Enema
Objective:To identify the rhubarb and salvia miltiorrhiza in Shenhuang detoxification enema by TLC and HPLC,to ensure the quality of enema in hospital and treat patients more effectively.Method:TLC uses silica gel plates,n-hexane-ethyl acetate-formic acid(40:10:0.5)and chloroform-acetone-formic acid(25:10:4)was the developing agent for Rhubarb and Salvia miltiorrhiza,respectively.And Rhubarb and Salvia miltiorrhiza as the reference medicinal materials,Chrysophanol and Sodium danshensu as the controls to compare the characteristic points of TLC.HPLC analyzed using a Kromasi lC18 chromatographic column(4.6 mm×250 mm,5 μm).The mobile phase of Rhein and Chrysophanol was methanol-0.1％phosphoric acid solution(85:15),the detection wavelength was 254 nm.The mobile phase of Tanshinone Ⅰ,Tanshinone Ⅱ A and Cryptotanshinone was acetonitrile-0.02％phosphoric acid solution(61:39),gradient elution,the detection wavelength is 270 nm.And the flow rateis 1.0mL/min,and thecolumn temperatureis 30℃.Results:The chromatographic spot separation of Shenhuang detoxification enema is good,and the characteristic spots of Rhubarb and Salvia miltiorrhiza are clear and consistent with the characteristic spots of the reference substance.Rhein,Chrysophanol,Tanshinone Ⅰ,Tanshinone Ⅱ A and Cryptotanshinone have a good linear relationship with the peak area within their respective ranges(r≥0.99),the average recovery rate of the added sample is 96.88％～108.28％,the RSD is 0.79％～2.76％,and the average content of Rhein and Tanshinone Ⅰ are(22.15±0.42)μg/Ml,(40.20±1.26)μg/Ml,respectively.Conclusion:This method has accurate results and good repeatability,we have formulated standards that the contents of Rhein and Tanshinone Ⅰ in Shenhuang detoxification enema should not be less than 15μg/Ml and 30μg/Ml,respectively,for quality control of Shenhuang detoxification enema.
Shenhuang detoxification enemaThin-layer chromatographyHigh-performance liquid chromatographyRhubarbSalvia miltiorrhizaquality control
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