The effect of sulfasalazine combined with Bacteroides fragilis treatment on the proportion of Treg in the spleen of mice with ulcerative colitis
Objective:To investigate the effect of sulfasalazine combined with Bacteroides fragilis treatment on the proportion of Treg in the spleen of mice with ulcerative colitis.Method:The study period was from January 1,2020 to December 31,2022.32 BALB/c mice were selected as the experimental subjects of this study.They were divided into a control group,a model group,a simple treatment group,and a combination group(sulfasalazine combined with Bacteroides fragilis)according to different treatment methods,with 8 mice in each group.The control group was not treated,while the model group,simple treatment group,and combination group were all treated with sodium glucose sulfate to produce chronic ulcerative colitis mice.The model group implemented modeling,while the simple treatment group received daily treatment with sulfasalazine on the basis of the model group,while the combination group received daily treatment with sulfasalazine and Vibrio fragilis on the basis of modeling.After 7 weeks of treatment,the mice were euthanized and their colon tissue was taken out for testing.The disease activity index(DAI),colon mucosal injury index(CMDI),A20 protein expression in colon tissue,and Treg ratio in spleen tissue were compared among the four groups.Result:Compared with the control group,the DAI score and CMDI score of the model group increased,and the relative expression level of A20 protein and the proportion of Treg decreased;Compared with the model group,the DAI score and CMDI score of the simple treatment group decreased,and the relative expression level of A20 protein and the proportion of Treg increased;Compared with the simple treatment group,the combination group showed a significant decrease in DAI and CMDI scores,and the relative expression level of A20 protein and Treg ratio increased.Conclusion:In the study of ulcerative colitis mice,it was found that treatment with sulfasalazine and Bacteroides fragilis had a high effect and could effectively improve the Treg ratio in the spleen tissue of mice.
ulcerative colitissulfasalazinefragile Bacteroidesdisease activity indexcolon mucosal injury indexA20 proteinTreg ratio in spleen tissue
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