Analysis and research on the influencing factors of membrane filtration method
Objective:To explore the effects of different cultivation methods,mixing time,and pre filtration on the recovery rate of membrane filtration method.Method:Referring to the sterile test method 1101 in the 2020 edition of the Chinese Pharmacopoeia,the membrane filtration method was used to conduct experiments on different cultivation methods,mixing times,and pre filtration media.Result:Coverage culture and conventional culture have different applicability to different bacterial strains.For bacterial strains that are prone to expanding and growing around during the cultivation process,coverage culture method can be preferred,but it is not suitable for some bacterial strains;Overall,the longer the mixing time,the lower the recovery rate;The recovery rate is highest when using dust-free cloth as the pre filtration medium.Conclusion:This study indicates that the selection of appropriate cultivation methods,mixing time,and pre filtration media have a significant impact on the recovery rate of the membrane filtration method.
万方数据
维普
