Determination of echinacoside in Biantong gel plaster by HPLC
Objective:To establish a method for the determination of echinacoside in Biantong gel plaster in order to better control the quality of Biantong gel plaster.Methods:Determination of Cistanche deserticola by HPLC according to Chinese Pharmacopoeia Ⅰ;Gradient elution was carried out with methanol as mobile phase A and 1％ acetic acid solution as mobile phase B.DikmaDiamonsil (Diamond C185μm250×4.6mm) was used as chromatographic column.The flow rate was 1mL·min-1 .The column temperature was 30℃. The sample injection amount was 20μL.The detection wavelength was 332nm.Results:The concentration of echinacoside has a good linear relationship in the range of 0.6μg·mL-1～45.0μg·mL-1 .The regression equation was y=11594x-516.3,R2=0.9990;The recoveries ranged from 100.96 to 98.96,RSD=1 .0％.Conclusion:This method meets the determination requirements and can be used for the determination of echinacoside in Biantong gel plaster.
Biantong gel plasterechinacosidecontent determinationHPLC
万方数据
维普
