目的:建立高效液相色谱串联质谱法(High-Performance Liquid Chromatography Tandem Mass Spectrometry,HPLC-MS/MS)检测精神病患者血清中的多种抗精神病类药物(Antipsychotic drugs,AD)的浓度.方法:以氯氮平(LDP)、去甲氯氮平(QJLDP)、利培酮(LPT)、帕潘立酮(PPLT)、齐拉西酮(QLXT)稳定同位素为内标,样品处理后进行HPLC-MS/MS上清液分析.结果:各药物携带污染率为-0.17％～2.04％;在各自质量浓度水平表现出现良好的内线性,相关系数均＞0.9900;测得批内、批间准确度和精密度,回收率及基质效应均符合性能验证要求.结论:HPLC-MS/MS体系在前处理操作上更加简便,且检测速度更快,具有更高的灵敏度和准确性.这种方法不仅提高了临床检测的效率,同时也为精神病患者的个体化治疗提供了有力的支持.
High performance liquid chromatography tandem mass spectrometry for detecting the concentration of various antipsychotic drugs and metabolites in the serum of psychiatric patients
To establish a high-performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS)method for detecting the concentration of various antipsychotic drugs(AD)in the serum of psychiatric patients.Method:Stable isotopes of chlorpromazine(LDP),norclozapine(QJLDP),risperidone(LPT),papazolidone(PPLT),and ziprasidone(QLXT)were used as internal standards.After sample treatment,HPLC-MS/MS supernatant analysis was performed.ResμLt:The contamination rate of each drug was-0.17％～2.04％;Good internal linearity was observed at their respective mass concentration levels,with correlation coefficients＞0.9900;The accuracy and precision,recovery rate,and matrix effect measured within and between batches meet the performance validation requirements.Conclusion:The HPLC-MS/MS system is more convenient in pre-treatment operations,with faster detection speed,higher sensitivity and accuracy.This method not only improves the efficiency of clinical testing,but also provides strong support for personalized treatment of psychiatric patients.
clozapineHigh performance liquid chromatography tandem mass spectrometrymental illnessblood drug concentration
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