Objective:To explore the rapid detection results of sulfonylurea in hypoglycemic proprietary Chinese medicine by thin layer chromatography(TIC)and test tube reaction.Methods:Hypoglycemic proprietary Chinese medicine was selected as the research object.Liquid phase mass spectrometry(LC-MS)was used as the gold standard to determine the sulfonylurea components in drugs.TIC test method and test-tube reaction method were used for rapid detection of all drugs,and the final detection results were compared to compare the sensitivity,specificity and coincidence rate of the two detection methods.Results:Compared with the two rapid detection methods,the coincidence rate of the test tube reaction method was higher(P＜0.05),although the sensitivity and specificity of the two methods were higher than that of the test tube reaction method,but there was no significant difference(P＞0.05).Conclusion:In the detection of the addition of sulfonylurea components in hypoglycemic Chinese patent medicines,the test tube reaction method has better results,which is more suitable for field inspection.
关键词
降糖类中成药/磺酰脲类成分/TIC/试管反应法/快速检测
Key words
hypoglycemic proprietary Chinese medicine/Sulfonylurea components/TIC/test tube reaction method/rapid detection
维普
万方数据