Objective:In this study,high-throughput transcriptome sequencing technology was used to sequence the transcriptome of Tetrastigma hemsleyanum and functionally annotate to identified glycosyltransferases related to flavonoid glycoside metabolism.It could provide the references for the molecular breeding of and glycosyltransferase resource development of Tetrastigma hemsleyanum.Method:The transcriptome of Tetrastigma hemsleyanum was performed using an Illumina HiSeq 4000 sequencer.Denovo assembly of the Tetrastigma hemsleyanum transcriptomes was performed using trinity assembly method.The gene sequences were compared with functional databases,including the Nr,GO,KOG,Swiss-Prot,and KEGG database.Blast 2 GO was used to annotation functional genes information of the Tetrastigma hemsleyanum,including the sequence characteristics of bioinformatics glycosyltransferases.Results:Tetrastigma hemsleyanum samples were sequenced by gene assembly,a total of 229 003 sequences were obtained,the longest length of the sequence is 17232 bp,the smallest length is 201 bp,the average length of the sequence is 536 bp,the total number of bases accounts for 41.82％of the total number of bases,and the N50 is 769 bp.After annotation,information Unigene got 125 101,and the highest number of annotated Unigene was Nr database with 123 549,followed by GO database.Four flavonoid metabolism pathways related to with a total of 760 genes,the glycosyltransferases annotated as crocetin glucosyltransferase,anthocyanidin 3-O-glucoside 5-O-glucosyltransferase,coniferyl-alcohol glucosyl transferase.Conclusion:In this paper,we performed high-throughput transcriptome sequencing and functional annotation of the sequences of Tetrastigma hemsleyanum to obtain the glycosyltransferases synthesis-related genes,and annotation of the flavonoid glycoside-related glycosyltransferases,which will provide reference value for the molecular breeding of Tetrastigma hemsleyanum and the rational utilization of glycosyltransferase resources.
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