首页|实验教学用植物活体荧光影像系统的研制

实验教学用植物活体荧光影像系统的研制

扫码查看
通过植物培养及拍摄系统的开发,研制低成本的植物活体荧光影像系统.该系统采用双层箱体结构设计,分为恒温光照培养系统和荧光记录系统两部分,可以为植物生长发育提供所需的生命支撑功能,还能实时获取植物生长发育过程中的关键图像,包括监控转基因植物发育过程中荧光信号的时空模式.利用该系统成功地对EGFP转基因烟草和拟南芥进行培养和实时观察.低廉的成本极大地扩展了该系统在实验教学中的运用,也为生命活动的观察和检测研究奠定基础.
Research and Development of Living Plants in-vivo Fluorescence Imaging System for Experimental Teaching
Through the development of plant culture and imaging system,a low-cost plant living fluorescence imaging system was developed.The system adopts a double-layer box structure design,which is divided into two parts:the constant culture system and the fluorescence recording system.It can provide the necessary life support function for plant growth,and can also obtain the key images in the process of plant growth in real time,including the time-space mode of monitoring the fluorescence signal in the development of the transgenic plant.The EGFP transgenic tobacco and Arabidopsis were successfully cultured and observed in real time using this system.The low cost greatly expanded the application of the system in experimental teaching,and also laid a foundation for the further study of the observation and detection of follow-up life activities.

instrument developmentin-vivo fluorescence imaging system for living plantsplant cultivation real-time microscope recorderplant incubator

王璠璘、贺新强、王东辉

展开 >

北京大学生物学国家级实验教学示范中心, 蛋白质与植物基因研究国家重点实验室, 北京大学生命科学学院,北京 100871

仪器研制 植物活体荧光影像系统 植物培养实时显微记录仪 植物培养箱

北京大学实验室与设备管理部教学改革经费资助

2024

北京大学学报(自然科学版)
北京大学

北京大学学报(自然科学版)

CSTPCD北大核心
影响因子:0.785
ISSN:0479-8023
年,卷(期):2024.60(1)
  • 5