蒲公英金纳米颗粒的制备及体外抗癌活性研究
Preparation and in vitro anticancer activity of dandelion gold nanoparticles
刘素素 1王文博 1李璋龙 1陈嘉程 1王银川 1喻长远 1刘佳蕙 2王诗卉1
作者信息
- 1. 北京化工大学 生命科学与技术学院,北京 100029
- 2. 北京大学 化学与分子工程学院 分析测试中心,北京 100871
- 折叠
摘要
以蒲公英提取液为还原剂、氯金酸为原料合成了蒲公英金纳米颗粒(Da-AuNPs),采用多种手段对其进行了表征,结果表明:Da-AuNPs呈球形或近球形,平均粒径约为(21.2±3.0)nm;蒲公英中含丰富羟基的活性物质可能是合成Da-AuNPs的主要还原剂;金纳米颗粒具有典型的面心立方(FCC)晶格结构;Da-AuNPs悬液放置5d,其粒径没有显著变化,体系具有较好的稳定性.采用噻唑蓝溴化四唑(MTT)试验对Da-AuNPs进行了体外细胞毒性测试,结果显示:在6.25～200 μg/mL的范围内,Da-AuNPs对RAW264.7 正常细胞具有良好的生物相容性;当Da-AuNPs的质量浓度为200 μg/mL时,在808 nm的近红外线(1.5 W/cm2)下照射3 min,与对照组相比4T1 癌细胞活力下降至65%,表明Da-AuNPs具有一定的体外抗癌活性.
Abstract
Dandelion gold nanoparticles(Da-AuNPs)were synthesized by using dandelion extract as a reducing a-gent and chloroauric acid as raw material and characterized by various methods.The results show that Da-AuNPs are spherical or nearly spherical,and the average particle size is(21.2±3.0)nm.The active substances contai-ning abundant hydroxyl groups in dandelions may be the main reducing agents for the synthesis of Da-AuNPs.Gold nanoparticles have a typical face-centered cubic(FCC)lattice structure.When a Da-AuNPs suspension was left for 5 days,its particle size did not change significantly,indicating that the system had good stability.The in vitro cytotoxicity of Da-AuNPs was tested by MTT assay.The results showed that Da-AuNPs had good biocompatibility to RAW264.7 normal cells in the range 6.25-200 μg/mL.After exposure to 808 nm near-infrared radiation(1.5 W/cm2)for 3 min,when the mass concentration of Da-AuNPs was200μg/mL,the viability of 4T1 cancer cells decreased to 65%compared with the control group,indicating that Da-AuNPs had a certain anti-cancer ac-tivity in vitro.
关键词
蒲公英/金纳米颗粒/绿色合成/抗癌活性Key words
dandelion/gold nanoparticles/green synthesis/anticancer activity引用本文复制引用
基金项目
国家自然科学基金(82174531/21606013)
国家重点研发计划(218YFA0903000)
出版年
2024
NETL
NSTL
万方数据